Supercharge Your Innovation With Domain-Expert AI Agents!

Application of tavq25 Gene in Regulating Resistance of Wheat to Powdery Mildew and Sheath Blight

A technology for sheath blight and powdery mildew, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as time-consuming, difficult to achieve shape-oriented improvement, difficult to achieve precise point mutation, etc., to achieve improved ability , the effect of significant application and promotion value

Active Publication Date: 2022-04-08
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional hybrid breeding is time-consuming, heavy workload, and it is difficult to achieve shape-oriented improvement; physical, chemical, biological and other mutagenesis methods are difficult to achieve precise point mutation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of tavq25 Gene in Regulating Resistance of Wheat to Powdery Mildew and Sheath Blight
  • Application of tavq25 Gene in Regulating Resistance of Wheat to Powdery Mildew and Sheath Blight
  • Application of tavq25 Gene in Regulating Resistance of Wheat to Powdery Mildew and Sheath Blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preparation of recombinant plasmids

[0048] Artificially synthesized recombinant plasmid pCXUN-Cas9-gRNA. The recombinant plasmid pCXUN-Cas9-gRNA is a circular plasmid. The nucleotide sequence of the recombinant plasmid pCXUN-Cas9-gRNA is shown in SEQ ID NO:1. In SEQ ID NO: 1, starting from the 5' end, the 115th-367th nucleotides are reverse complementary to the NOS terminator, the 392nd-4522nd nucleotides are reverse-complementary to the coding gene of the Cas9 protein, and the 4544-6533rd The first nucleotide is reverse complementary to the Ubi promoter, the 6552-6914th nucleotide is the U6 promoter, and the 6915-7017th nucleotide is the coding gene of the sgRNA (the 6915-6934th nucleotide is the target sequence recognition region). The recombinant plasmid pCXUN-Cas9-gRNA expresses sgRNA, and the target sequence of the sgRNA is located in the wheat TaVQ25 gene, and the specific target sequence is shown in SEQ ID NO:8.

[0049] In the wheat cDNA: the coding reg...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of TaVQ25 gene in regulating the resistance of wheat to powdery mildew and sheath blight. The gene is TaVQ25 related to jasmonic acid signal conduction, and encodes TaVQ25 protein. The invention adopts CRISPR / Cas9 technology to knock out the wheat TaVQ25 gene, and obtains new wheat germplasm with significantly improved resistance to powdery mildew and sheath blight. The invention improves the ability of wheat to resist powdery mildew and sheath blight, innovates germplasm resources of wheat disease resistance, is of great significance to new variety cultivation, environmental sanitation and food safety, and has great application and popularization value.

Description

technical field [0001] The invention relates to the application of TaVQ25 gene in regulating the resistance of wheat to powdery mildew and sheath blight in the field of biotechnology. Background technique [0002] VQ proteins are a class of plant-specific proteins, all of which have a conserved VQ-motif: FxxxVQx(L / V / F)TG, where x is any amino acid. In 2002, VQ protein was discovered for the first time in Arabidopsis thaliana. At present, this kind of protein has been found and identified in wheat, cotton, corn, grape, soybean and other plants. Studies on the function of VQ have found that it not only participates in the regulation of various life processes of plants, but also participates in the response of plants to biotic and abiotic stresses. Due to the large wheat genome and allohexaploidity, the identification and biological functions of wheat VQ members need further study. [0003] Wheat powdery mildew is an epidemic disease caused by wheat powdery mildew (Blumeria g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H5/10A01H6/46
CPCC07K14/415C12N15/8218C12N15/8282
Inventor 夏兰琴田金福李少雅
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com