Wickerhamomyces anomalus strain for whole-process green production of fruit wine, and application of wickerhamomyces anomalus strain

A Wickham and abnormal technology, applied in the field of abnormal Wickham yeast, can solve the problem of not effectively reducing the taste of fruit wine organic sour fruit wine, and achieve the effect of preventing fresh fruit from rotting, increasing content, and ensuring freshness

Active Publication Date: 2020-12-18
山东凯普菲特生物科技有限公司
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  • Abstract
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Problems solved by technology

But lactic acid is also an organic acid, so this method can not effectively re

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  • Wickerhamomyces anomalus strain for whole-process green production of fruit wine, and application of wickerhamomyces anomalus strain
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  • Wickerhamomyces anomalus strain for whole-process green production of fruit wine, and application of wickerhamomyces anomalus strain

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[0035] In order to better explain the present invention and facilitate understanding, the present invention will be described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0036] 1. Screening and identification of strains

[0037] (1) Isolation of strains

[0038] The naturally fermented blueberry fruit wine was sampled on 0d, 3d, 6d, 9d, 12d and 15d respectively, and the wine sample liquid was diluted 1000 times, 10000 times and 100000 times in Bengal red medium, and the samples were plated, and after 48 hours of constant temperature incubation at 26°C A single colony was obtained, and all experiments were performed in duplicate. Pick a single colony on the Bengal red medium plate for isolation and purification, culture at a constant temperature of 26°C for 48 hours, pick suspected colonies according to the morphological characteristics of the yeast for microscopic examination, and separate them by streaking on the Bengal red m...

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Abstract

The invention relates to a wickerhamomyces anomalus strain. The wickerhamomyces anomalus strain is preserved in the China general microbiological culture collection centre on April 24th, 2020; the preservation number of the wickerhamomyces anomalus strain is CGMCC No.19723; and the wickerhamomyces anomalus strain is named as Wickerhamomyces anomalus WTB20042303. The strain can effectively utilizecitric acid and tartaric acid, has degradation capacity on citric acid, lactic acid, formic acid, acetic acid and succinic acid in the fruit wine, and particularly has remarkable degradation capacityon citric acid; and the strain can effectively improve the content of active matters, such as total phenols, total flavonoids and anthocyanin, and improve the antioxidant level of the fruit wine. In addition, the strain also has a biocontrol effect superior to that of an existing known biocontrol strain, and has a remarkable inhibition effect on botrytis cinerea. Therefore, the strain can be applied to postharvest storage and preservation of fruits or deacidification and fermentation of fruit wine, and is a preferred strain, which can be applied to the whole process of a fruit wine preparationprocess.

Description

technical field [0001] The invention relates to a strain of functional yeast, in particular to a strain of abnormal Wickham yeast used in the whole process of green production of fruit wine and its application. Background technique [0002] Fruit wine is the wine that yeast uses the sugar of the fruit itself to ferment into alcohol. It contains the specific flavor and nutrients of the fruit. It is the wine that humans first learned to brew. There are all kinds of fruits that can be used to make fruit wine, among which kiwi, red bayberry, orange, grape, blueberry, red date, cherry, lychee, peach, persimmon, strawberry, etc. are more ideal. Among the fruit wines, wine is the most famous. In recent years, blueberries are very popular because of their rich anthocyanins, which can fight against free radicals and have the function of delaying aging. [0003] The brewing process of fruit wine is: picking fresh fruit → sorting → crushing, destemming → pulp → separation and extracti...

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Application Information

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IPC IPC(8): C12N1/16C12G3/024A23B7/155A23B7/16C12R1/645C12R1/865
CPCC12G3/024A23B7/155A23B7/16A23V2002/00C12R2001/645C12N1/145A23V2250/76A23V2200/10
Inventor 王友升
Owner 山东凯普菲特生物科技有限公司
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