Preparation and application of coral snake bitten pig model

Abstract

The invention discloses preparation and application of a coral snake bitten pig model. Coral snake venom freeze-dried powder is injected into pig muscles, the coral snake bitten pig model is established, and the defect that no experimental animal model exists for coral snake bites is overcome. The model is close to the characteristic that the disease course of human beings bitten by coral snakes is long, can simulate the coral snake bite process, can be used for researching clinical characterization, pathophysiological mechanism and precise treatment of the incidence of coral snake bites, andcan finally evaluate the curative effects of anti-snake venom serum and anti-venomous snake bite drugs.

Description

technical field

[0001] The invention belongs to the field of medicine, and in particular relates to the preparation and application of a pig model bitten by a silver ring snake. Background technique

[0002] Venomous snake bites (snakebites) are listed by the World Health Organization as one of the important tropical / subtropical diseases that are easily overlooked. There are about 5 million snakebites in the world every year, resulting in about 25,000-125,000 deaths. The number of patients with snakebite in my country reaches as many as 300,000 every year, the mortality rate is 5%-10%, and the disabled or disabled persons account for 25%-30%, which brings a great burden to families and society. According to epidemiological surveys, bites from snakebites in my country account for 8.12% of all kinds of venomous snake bites, but the number of deaths ranks first in snakebite incidents. Corona snake bite is an acute and severe disease, mainly manifested as drowsiness, motor nerv...

Examples

Embodiment 1

[0014] Embodiment 1: the preparation of the pig model of Bungare bite

[0015] 1. To explore the MLD of intramuscular injection of Bungara venom in pigs: 18 piglets, male or female, weighing about 10kg were purchased from the School of Animal Sciences of Guangxi University, and divided into five groups with different concentrations of Bungare venom and one control group. Bungare venom was injected at 0.2ml / kg in the outer muscle of the left leg of the piglet. The concentration was designed according to the theoretical half-lethal dose. The concentration gradient of Bungare venom was set according to 2 times the interval. The concentration gradient of Bungare venom was 240, 120, 60, 30, 15 μg / kg, repeat the concentration of three individuals in each group, inject the same amount of saline to 3 piglets in the control group, observe the survival status and biological behavior of the pigs, and explore the intramuscular injection of silver ring in piglets The minimum lethal dose o...

Embodiment 2

[0029] Embodiment 2: Application of Bungalia biting pig model

[0030] The pig model bitten by the silver ring snake established by the invention makes up for the deficiency that there is no experimental animal model for the bite of the silver ring snake. The model is close to the long course of human being bitten by the coral snake. It can simulate the process of the bite of the snake. It can be used to study the clinical manifestations, pathophysiological mechanism, and precise treatment of the disease, and can finally evaluate antivenom and antivenom. Efficacy of antivenomous snakebite drugs.

technical field

[0001] The invention belongs to the field of medicine, and in particular relates to the preparation and application of a pig model bitten by a silver ring snake. Background technique

[0002] Venomous snake bites (snakebites) are listed by the World Health Organization as one of the important tropical / subtropical diseases that are easily overlooked. There are about 5 million snakebites in the world every year, resulting in about 25,000-125,000 deaths. The number of patients with snakebite in my country reaches as many as 300,000 every year, the mortality rate is 5%-10%, and the disabled or disabled persons account for 25%-30%, which brings a great burden to families and society. According to epidemiological surveys, bites from snakebites in my country account for 8.12% of all kinds of venomous snake bites, but the number of deaths ranks first in snakebite incidents. Corona snake bite is an acute and severe disease, mainly manifested as drowsiness, motor nerv...

Examples

Embodiment 1

[0014] Embodiment 1: the preparation of the pig model of Bungare bite

[0015] 1. To explore the MLD of intramuscular injection of Bungara venom in pigs: 18 piglets, male or female, weighing about 10kg were purchased from the School of Animal Sciences of Guangxi University, and divided into five groups with different concentrations of Bungare venom and one control group. Bungare venom was injected at 0.2ml / kg in the outer muscle of the left leg of the piglet. The concentration was designed according to the theoretical half-lethal dose. The concentration gradient of Bungare venom was set according to 2 times the interval. The concentration gradient of Bungare venom was 240, 120, 60, 30, 15 μg / kg, repeat the concentration of three individuals in each group, inject the same amount of saline to 3 piglets in the control group, observe the survival status and biological behavior of the pigs, and explore the intramuscular injection of silver ring in piglets The minimum lethal dose o...

Embodiment 2

[0029] Embodiment 2: Application of Bungalia biting pig model

[0030] The pig model bitten by the silver ring snake established by the invention makes up for the deficiency that there is no experimental animal model for the bite of the silver ring snake. The model is close to the long course of human being bitten by the coral snake. It can simulate the process of the bite of the snake. It can be used to study the clinical manifestations, pathophysiological mechanism, and precise treatment of the disease, and can finally evaluate antivenom and antivenom. Efficacy of antivenomous snakebite drugs.

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