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Preparation method of high-activity lactic acid bacteria

A technology with high activity of lactic acid bacteria, applied in the direction of sterilization methods, biochemical equipment and methods, methods of supporting/immobilizing microorganisms, etc., can solve the problems affecting the cultivation efficiency and effect of lactic acid bacteria, and the quality of lactic acid bacteria is not up to standard, so as to reduce the The effect of uneven distribution of strains, increasing the number of cultures, and improving stability

Inactive Publication Date: 2021-02-02
孙震颖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a technical solution, which can effectively solve the problem that the solid-state culture medium used by lactic acid bacteria in the above-mentioned background technology is generally relatively large, requiring staff to use equipment to crush it in advance, and the crushed solid-state culture medium will still contain relatively large particles , it is easy to affect the cultivation efficiency and cultivation effect of lactic acid bacteria, resulting in the problem that the quality of the prepared lactic acid bacteria is not up to standard

Method used

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  • Preparation method of high-activity lactic acid bacteria
  • Preparation method of high-activity lactic acid bacteria
  • Preparation method of high-activity lactic acid bacteria

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Embodiment

[0050] Example: such as figure 1 Shown, the present invention provides technical scheme, a kind of highly active lactic acid bacteria preparation method, comprises the steps:

[0051] S1. Culture medium preparation: select the MRS solid medium, use the grinding and feeding mechanism 10 to grind it, add it into the culture chamber together with additives, and prepare a medium;

[0052] S2, medium pretreatment: select the prepared medium for homogenization treatment, so that the solid medium in the medium and the additive are evenly mixed;

[0053] S3. Preheating the culture tank: using the layered water bath insulation mechanism 8 above the top of the substrate 1 to preheat the culture tank layer by layer;

[0054] S4. Activation and addition of strains: select lactic acid bacteria strains for activation, then add the activated strains into the cultivation tank, and use the deodorization discharge mechanism 11 to deodorize and discharge the gas in the cultivation tank;

[005...

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Abstract

The invention discloses a preparation method of high-activity lactic acid bacteria. The method comprises the following steps: preparing a culture medium, pretreating the culture medium, preheating a culture tank, activating and adding strains, culturing the lactic acid bacteria, carrying out enlarged culture on the lactic acid bacteria, disinfecting and cleaning. A culture box is fixedly installedat the top end of a base plate, a cleaning box is fixedly installed at the position, corresponding to one side of the culture box, of the top end of the base plate, a control switch is installed at one end of the culture box in an embedded mode, and a deodorizing top cover is installed at the top end of the culture box. The structure is scientific and reasonable, use is safe and convenient, by arranging a return pump, heating pipes, a water inlet cavity, a water outlet cavity, a water outlet pipe and placing annular plates, the culture tank can be preheated in different areas, different lactic acid bacteria can be cultured at constant temperature in different areas, various lactic acid bacteria can be cultured at a time, the applicability of lactic acid bacteria culture is wider, the efficiency and effect of lactic acid bacteria culture are improved, the total consumed time of culture of different types of lactic acid bacteria is shortened, and the economy of lactic acid bacteria culture is improved.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria preparation, in particular to a method for preparing highly active lactic acid bacteria. Background technique [0002] Lactic acid bacteria is a general term for a class of bacteria that can use fermentable carbohydrates to produce a large amount of lactic acid. This type of bacteria is widely distributed in nature and has rich species diversity. Except for a few, most of the lactic acid bacteria are essential in the human body They are not only ideal materials for studying classification, biochemistry, genetics, molecular biology and genetic engineering, but also have important academic value in theory. The application value in important fields closely related to human life such as industry, agriculture and animal husbandry, food and medicine is also extremely high; [0003] At present, when the preparation method of soft acid bacteria is implemented, the solid medium used by lactic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12M1/02C12M1/12C12M1/00B01D53/04
CPCB01D53/04B01D2253/102B01D2257/80C12M21/16C12M23/34C12M23/48C12M27/16C12M29/00C12M37/00C12M39/00C12M41/22C12M45/02C12M47/18C12N1/20
Inventor 孙震颖
Owner 孙震颖
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