Fructus schisandrae and rhodotorula mucilaginosa fermentation extract as well as preparation method and application thereof
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A technology of red yeast and schisandra, which is applied in the field of fermented extract of red yeast schisandra and its preparation, can solve the problems of high risk, skin irritation, and low recovery rate of water extraction, and achieve the effect of low inoculation amount and reduced adsorption
Active Publication Date: 2021-02-05
肽源(广州)生物科技有限公司
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[0004] The extraction process of Schisandra chinensis is mainly based on organic solvent extraction. The traditional extraction process is based on water or organic solvent extraction. Water extraction has a low recovery rate, and organic solvent extraction has environmental and safety considerations.
Moreover, this kind of plant extracts generally has allergens, which have certain irritation to the skin, and there is a greater risk.
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Embodiment 1
[0027] (1) Take 100g of Schisandra chinensis, mix it with 1000g of water evenly after pulverization, pasteurize it, cool it to room temperature, and add pectinase to obtain a crushed Schisandra chinensis liquid; the amount of added pectinase is 0.01% of the mass of Schisandra chinensis crushed liquid;
[0028] (2) Inoculate 1, 2, 4, 6, 8, 12ml (1×10 6 CFU / ml), cultivated at 25°C for 48 hours to obtain the Schisandra Rhodotorula fermented liquid fermented with different inoculum amounts;
[0029] (3) Schisandra Rhodotorula fermented liquid was warmed up to 50° C., incubated for 3 hours, then centrifuged, and the centrifuged supernatant was filtered with a 0.22 μm filter membrane to obtain fermented extracts with different inoculum sizes.
Embodiment 2
[0031] (1) Take 100 g of Schisandra chinensis, mix it with 300 g of water after crushing, pasteurize and cool to room temperature and add pectinase to obtain a crushed Schisandra chinensis liquid; the addition of the pectinase is 0.01% of the mass of the Schisandra chinensis crushed liquid;
[0032] (2) Get above-mentioned and obtain pulverizing liquid 1g, add in 200ml basal fermentation medium; The prescription of basal fermentation medium comprises the composition of following mass percent: glucose 5%, (NH 4 ) 2 SO 4 0.1%, KH 2 PO 4 0.1%, CaCl 2 0.01%, NaCl 0.04%, MgSO 4 ·7H 2 O 0.1%, pH adjusted to 6.5;
[0033] (3) Mix 10ml (1×10 6 CFU / ml) Rhodotorula gluei seeds were inserted into the above-mentioned medium, cultured for 24 hours, centrifuged to sample the supernatant for detection.
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Abstract
The invention belongs to the technical field of cosmetic raw materials, and discloses a fructus schisandrae and rhodotorula mucilaginosa fermentation extract and a preparation method and application thereof. The preparation method comprises the following steps of (1) crushing fructus schisandrae, uniformly mixing the crushed fructus schisandrae with water according to a mass ratio of 1: 2-1: 10, carrying out pasteurization, cooling to room temperature, and adding pectinase to obtain crushed fructus schisandrae liquid; activating and amplifying rhodotorula mucilaginosa, adding the rhodotorula mucilaginosa into the crushed fructus schisandrae liquid, and adding 1*10<6> to 1* 10<9> live rhodotorula mucilaginosa thalli into each kilogram of the crushed fructus schisandrae liquid; conducting culture for 24-72 hours at the temperature of 20-32 DEG C to obtain fructus schisandrae and rhodotorula mucilaginosa fermentation liquor; and heating the fructus schisandrae and rhodotorula mucilaginosafermentation liquor to 40-60 DEG C, keeping the temperature for 28 hours, afterwards conducting centrifuging, and filtering the centrifuged supernatant by using a 0.22-micrometer filter membrane to obtain the fructus schisandrae and rhodotorula mucilaginosa fermentation extract. According to the method, the good antibacterial effect of fructus schisandrae is reserved, the oxidation resistance isimproved, and the irritation is reduced.
Description
technical field [0001] The invention belongs to the technical field of cosmetic raw materials, in particular to a schisandra rhodotorula fermented extract and its preparation method and application. Background technique [0002] Schisandra is the dried and mature fruit of Magnoliaceae Schisandra [Schisandra chinensis (Turcz.) Bailll] and Schisandra sphenanthera Rehd et Wils. The former is called Northern Schisandra, and the latter is called Southern Schisandra. Chinese herbal medicine. Pharmacological studies have shown that in addition to sedative, hypnotic, and anticonvulsant effects, Schisandra chinensis also has the effects of strengthening the heart, protecting the liver, inhibiting bacteria, anti-tumor, anti-oxidation, and delaying aging. [0003] Rhodotorula gelatinous yeast is widely distributed in nature, and it can be found in soil, animals, plants, rivers, lakes and other fresh waters and saline marine environments. Rich in nutrients, in addition to being rich i...
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