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Application of benidipine in the preparation of drugs against novel coronavirus infectious diseases

An infectious disease, coronavirus technology, applied in the field of medicine to achieve strong anti-coronavirus effect

Active Publication Date: 2021-08-13
CANVEST WUHAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no effective antiviral drugs and vaccines for the treatment of COVID-19, mostly symptomatic and supportive treatment

Method used

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  • Application of benidipine in the preparation of drugs against novel coronavirus infectious diseases
  • Application of benidipine in the preparation of drugs against novel coronavirus infectious diseases
  • Application of benidipine in the preparation of drugs against novel coronavirus infectious diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment studies the cytotoxicity of the target compound benidipine hydrochloride, and the specific process is as follows:

[0030] Vero E6 cells were divided into 1.5 × 10 4 Cells / well were seeded in 96-well cell culture plates at 37°C, 5% CO 2 After culturing in the incubator for 12-16 hours, replace the medium with DMEM medium containing 2% FBS, and then use Treat with benidipine hydrochloride, add the same volume of DMSO to the negative control, set up three duplicate wells for each concentration, incubate at 37°C for 24 hours, and set up zero-adjustment wells with only medium and no cells, and use the CCK8 detection kit to detect cell viability. Test results such as figure 1 shown, and calculated the CC of benidipine hydrochloride on Vero E6 cells by drawing the cell viability curves under different concentrations 50 is >100 μM.

Embodiment 2

[0032] In this embodiment, the antiviral activity of the target compound benidipine hydrochloride on the new coronary pneumonia virus is studied, and the specific process is as follows:

[0033] Vero E6 cells were divided into 1.5 × 10 4 Cells / well were seeded in 48-well cell culture plates at 37°C, 5% CO 2 After culturing in the incubator for 12-16 hours, remove the supernatant from the cells in the 48-well plate, and then treat them with benidipine hydrochloride at a concentration of 0.164 μM, 0.49 μM, 1.48 μM, 4.44 μM, 13.3 μM and 40 μM, and incubate for 1 hour , add SARS-CoV-2 in a biosafety level 3 (BSL-3) laboratory, the multiplicity of infection (MOI) is 0.05, after incubation for 1 hour, remove the supernatant, wash with PBS, add the concentration of 0.164μM, 0.49μM , 1.48 μM, 4.44 μM, 13.3 μM and 40 μM benidipine hydrochloride, and the supernatant was collected 24 hours after infection. Collect 150ul of supernatant from each well and add it to 271ul of lysate for in...

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Abstract

The invention provides an application of benidipine in the preparation of drugs against novel coronavirus infectious diseases. The invention discovered for the first time that benidipine can be used to prevent and treat novel coronavirus infectious diseases, and by detecting the cytotoxicity of benidipine hydrochloride on VeroE6 cells and its antiviral activity against new coronary pneumonia virus, it shows that benidipine hydrochloride Dipin showed a strong anti-COVID-19 effect at the cellular level, providing a new direction for the prevention and treatment of COVID-19 infectious diseases.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of benidipine in the preparation of drugs against novel coronavirus infectious diseases. Background technique [0002] Benidipine chemical name: (±)-2,6,-dimethyl-4-(3-nitrophenyl)-1,4-dihydro-3,5-pyridinedicarboxylic acid, 3-(1- Benzyl-3-piperidinyl) ester-5-methyl ester, which was developed by Kyowa Hakko in 1981, and was approved by Food and Drug Administration (FDA) in 1991 ) approved and put into clinical use. For decades, benidipine has been effective in the treatment of essential hypertension, angina pectoris and other diseases. [0003] New Coronary Pneumonia (COVID-19) is an infectious disease caused by a new type of coronavirus (SARS-CoV-2). Its symptoms mainly include fever, fatigue, dry cough, dyspnea, and renal failure. As of May 18, 2020 The number of confirmed cases of new coronary pneumonia (COVID-19) caused by the new coronavirus ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4545A61P11/00A61P31/14
CPCA61K31/4545A61P11/00A61P31/14
Inventor 徐国东袁冰刘晓萍
Owner CANVEST WUHAN BIOTECH
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