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Application of clofazimine to preparation of medicines for resisting novel coronavirus infectious diseases

A technology for infectious diseases, coronavirus, applied in the field of medicine

Inactive Publication Date: 2021-02-09
CANVEST WUHAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] New Coronary Pneumonia (COVID-19) is an infectious disease caused by a novel coronavirus (SARS-CoV-2). Its symptoms mainly include fever, fatigue, dry cough, dyspnea, and renal failure. Viral drugs and vaccines are used in the treatment of COVID-19, mostly symptomatic and supportive treatment

Method used

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  • Application of clofazimine to preparation of medicines for resisting novel coronavirus infectious diseases
  • Application of clofazimine to preparation of medicines for resisting novel coronavirus infectious diseases
  • Application of clofazimine to preparation of medicines for resisting novel coronavirus infectious diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment studies the cytotoxicity of the target compound clofazimine, and the specific process is as follows:

[0025] Vero E6 cells were divided into 1.5 × 10 4 Cells / well were seeded in 96-well cell culture plates at 37°C, 5% CO 2 After culturing in the incubator for 12-16 hours, replace the medium with DMEM medium containing 2% FBS, and then use Clofazimine was used for treatment, and the same volume of DMSO was added to the negative control. Three replicate wells were set up for each concentration, and incubated at 37°C for 24 hours. At the same time, it was necessary to set up zero-adjustment wells with only medium and no cells, and use the CCK8 detection kit to detect cell viability. Test results such as figure 1 shown, and the CC of clofazimine on Vero E6 cells was calculated by plotting the cell viability curves at different concentrations 50 is >100 μM.

Embodiment 2

[0027] In this example, the antiviral activity of the target compound clofazimine on the new coronary pneumonia virus is studied, and the specific process is as follows:

[0028] Vero E6 cells were divided into 1.5 × 10 4 Cells / well were seeded in 48-well cell culture plates at 37°C, 5% CO 2 After culturing in the incubator for 12-16 hours, remove the supernatant from the cells in the 48-well plate, and then treat them with clofazimine at a concentration of 0.03 μM, 0.1 μM, 0.33 μM, 1 μM, 3.3 μM and 10 μM, and incubate for 1 hour. Add SARS-CoV-2 in a biosafety level 3 (BSL-3) laboratory, and the multiplicity of infection (MOI) is 0.05. After incubation for 1 hour, remove the supernatant, wash with PBS, and add concentrations of 0.033 μM, 0.1 μM, 0.33 μM, 1 μM, 3.3 μM and 10 μM of clofazimine, and the supernatant was collected 24 hours after infection. Collect 150 μl of supernatant from each well and add it to 271 μl of lysate for inactivation, soak the inactivated sample tub...

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Abstract

The invention provides an application of clofazimine to preparation of medicines for resisting novel coronavirus infectious diseases. The inventor finds for the first time that the clofazimine can beused for preventing and treating the novel coronavirus infectious diseases, the detection result on cytotoxicity of the clofazimine on VeroE6 cells and antiviral activity of the clofazimine on novel corona pneumonia viruses indicates that the clofazimine shows a high effect of resisting novel corona pneumonia viruses at the cellular level, and a new direction is provided for prevention and treatment of new corona pneumonia virus infectious diseases.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of clofazimine in the preparation of drugs against novel coronavirus infectious diseases. Background technique [0002] Clofazimine (clofazimine), also known as chlorophenazine, its chemical name is 10-(p-chlorophenyl)-2,10-dihydro-3-(p-chloroanilino)-2-isopropylidene Aminophenazine, the drug has inhibitory effect on Bacillus leprae. Its mechanism of action is to interfere with nucleic acid metabolism and inhibit bacterial protein synthesis. The effect is slower than that of dapsone. Currently, it is mainly used to treat the infection of Bacillus leprae resistant to dapsone. disease. [0003] New Coronary Pneumonia (COVID-19) is an infectious disease caused by a novel coronavirus (SARS-CoV-2). Its symptoms mainly include fever, fatigue, dry cough, dyspnea, and renal failure. Viral drugs and vaccines are used in the treatment of COVID-19, mostly sym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/498A61P31/14A61P11/00
CPCA61K31/498A61P11/00A61P31/14
Inventor 徐国东幸晓莹刘晓萍
Owner CANVEST WUHAN BIOTECH
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