Application of phosphodiesterase inhibitor or pharmaceutical composition thereof in preparation of medicines for treating novel coronavirus pneumonia

A phosphodiesterase, coronavirus technology, applied in the field of medicine

Pending Publication Date: 2022-02-18
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the above drugs for the treatment of new coronavirus pneumonia

Method used

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  • Application of phosphodiesterase inhibitor or pharmaceutical composition thereof in preparation of medicines for treating novel coronavirus pneumonia
  • Application of phosphodiesterase inhibitor or pharmaceutical composition thereof in preparation of medicines for treating novel coronavirus pneumonia
  • Application of phosphodiesterase inhibitor or pharmaceutical composition thereof in preparation of medicines for treating novel coronavirus pneumonia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] In this example, a preliminary evaluation of the effect of zadaverine on the novel coronavirus is carried out as follows:

[0045] (1) Use the following methods to study the impact of the novel coronavirus on the transcriptome of host cell lines:

[0046] 1. Experimental operation and analysis process

[0047] (1) In vitro experiment of viral infection of Huh7 (human liver cancer cells).

[0048] Huh-7 cells use DMEM+10% FBS medium, 37°C, 5% CO 2 standard environment. The cell suspension digested with trypsin was plated on a 12-well plate and cultured overnight. Discard the cell culture medium in the well the next day and replace it with a virus-containing medium (0.05 MOI). After incubation for 1 hour, discard the virus-containing medium, rinse with PBS three times, and replace with a standard medium. Lysed cells were harvested with TRIZOL at 3, 18, and 24 hours after infection. Transcriptome sequencing and analysis of Huh7 cells after virus infection.

[0049] (...

Embodiment 2

[0067] In this example, the in vitro inhibitory effect of zadavirin on the activity of the new coronavirus was determined by a cell-level experiment. And select other 3 phosphodiesterase inhibitors to verify the anti-new coronavirus activity.

[0068] 1. Experimental operation and analysis process

[0069] Six treatment concentrations (100 μM, 33.3 μM, 11.1 μM, 3.7 μM, 1.23 μM, 0.41 μM) were set for each drug, and a non-infected cell control and an infection control without drug treatment were set on each plate. Huh-7 cells use DMEM+10% FBS medium, 37°C, 5% CO 2 standard environment. The cell suspension after trypsinization (Huh7 cells were inoculated at 2.1×104 / 100 μL) was spread on a 12-well plate and cultured overnight. Discard the cell culture medium in the well the next day and replace it with virus-containing medium (0.05 multiplicity of infection). After incubation for 1 hour, discard the virus-containing medium, rinse with PBS three times, and replace with standard ...

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PUM

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Abstract

The invention provides the application of a phosphodiesterase inhibitor or a pharmaceutical composition of the phosphodiesterase inhibitor in preparation of medicines for treating novel coronavirus pneumonia, screening of systematic biology and cellular level experiments verify that zardaverine, Pimobendan and crisaborole can inhibit proliferation of novel coronavirus to different degrees on the cellular level; wherein zardaverine has the strongest anti-new coronavirus effect, Pimobendan has the second anti-new coronavirus effect, and zardaverine, Pimobendan and crisaborole can be used for preparing medicines for treating novel coronavirus pneumonia.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of phosphodiesterase inhibitors or pharmaceutical compositions thereof in the preparation of medicines for the treatment of novel coronavirus pneumonia, in particular to the phosphodiesterase inhibitors zadavirine, pimobendan, and clindamycin Application of bororo or its pharmaceutical composition in the preparation of medicines for treating novel coronavirus pneumonia. Background technique [0002] The global pandemic of novel coronavirus pneumonia in 2020, as of July 27, the number of confirmed patients worldwide has exceeded 16.26 million, which has had an important impact on the lives and health of people all over the world and the development of the world economy. In my country, although the epidemic has been generally contained, there are still risks of small-scale epidemics and repeated outbreaks. [0003] COVID-19 has been a global pandemic for several months, yet n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/50A61K31/501A61K31/69A61P11/00A61P31/14
CPCA61K31/50A61K31/501A61K31/69A61P11/00A61P31/14A61K2300/00
Inventor 胡宇慧赵金存李云飞孙静冯若轻赵研
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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