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Analytical system and analytical method thereof

An analysis method and analysis system technology, applied in biostatistics, instrumentation, hybridization, etc., can solve problems such as unfavorable detection, low excitation efficiency, cross-interference, etc.

Pending Publication Date: 2021-02-23
CREDO DIAGNOSTICS BIOMEDICAL PTE LTD
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  • Abstract
  • Description
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Problems solved by technology

However, signals in adjacent frequency bands will cause crosstalk problems, and crosstalk problems will change with solution concentration or temperature, which will affect the accuracy and efficiency of analysis
Furthermore, the excitation efficiency of the analyte excited by a wide-band xenon lamp (Xenon light) is low, so it is not conducive to the detection of low-concentration analytes or a variety of analytes, and it is necessary to configure a complex and bulky optical system

Method used

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  • Analytical system and analytical method thereof
  • Analytical system and analytical method thereof
  • Analytical system and analytical method thereof

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Embodiment Construction

[0030] Please refer to figure 1 , figure 1 It is a schematic diagram of the analysis system 10 in the embodiment of the present invention. The analysis system 10 can be used to detect and analyze fluorescent signals in real time. The analysis system 10 includes a light source 100 , optical elements 110 , 130 , a test object 120 , a detector 140 , a processing circuit 150 and a storage device 160 . The light beam LB emitted by the light source 100 is directed to the object under test 120 through the optical element 110 and excites the object under test 120 to generate a fluorescence signal FS (also referred to as a first fluorescence signal). The fluorescence signal FS can be directed to the detector 140 through the optical element 130 , and the detector 140 receives the fluorescence signal FS and measures the corresponding relationship between (light) intensity and wavelength of the fluorescence signal FS, and outputs the measured data DTr to the processing circuit 150 . Th...

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Abstract

The invention provides an analytical system and an analytical method thereof. The analytical method includes obtaining a first data and performing curve fitting on the first data according to at leastone basis to generate at least one coefficient corresponding to the at least one basis, wherein a function pattern corresponding to one of the at least one basis has asymmetry. The analytical systemand analytical method can improve analysis accuracy and analysis efficiency, and are beneficial to miniaturization.

Description

technical field [0001] The present invention refers to an analysis system and its analysis method, especially to an analysis system and its analysis method with high analysis accuracy and analysis efficiency, which is beneficial to size miniaturization. Background technique [0002] Polymerase chain reaction (polymerase chain reaction, PCR) DNA amplification reaction is an important technique in molecular biology. Wherein, real-time polymerase chain reaction refers to performing DNA amplification reaction and quantitative analysis after DNA amplification simultaneously in the same sample container. Quantitative analysis after DNA amplification is to separate the excited fluorescent signals of the analytes into different multiple optical paths, and filter them into multiple signals of different frequency bands through multiple bandpass filters for analysis. However, signals in adjacent frequency bands will cause crosstalk problems, and the crosstalk problems will change with...

Claims

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Application Information

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IPC IPC(8): G16B40/10G16B25/20
CPCG16B40/10G16B25/20G16B40/20G16B40/30
Inventor 赖盈达欧育诚陈依希陈冠颖陈玺元刘育佑
Owner CREDO DIAGNOSTICS BIOMEDICAL PTE LTD
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