Single-cell mass spectrometry system and method based on parallel processing technology

A mass spectrometry analysis and parallel processing technology, applied in the field of cell analysis, can solve the problems of long process waiting, low analysis efficiency, not considering cleaning, etc., and achieve the effect of ensuring accuracy, accurate analysis results, and good cleaning effect.

Active Publication Date: 2021-03-05
NINGBO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The analysis efficiency is low, and each functional module is executed sequentially, and more time is spent waiting for the process; in addition, most single-cell mass spectrometry devices do not consider cleaning, and there is a possibility of cross-contamination

Method used

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  • Single-cell mass spectrometry system and method based on parallel processing technology
  • Single-cell mass spectrometry system and method based on parallel processing technology
  • Single-cell mass spectrometry system and method based on parallel processing technology

Examples

Experimental program
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Effect test

Embodiment 1

[0031] figure 1 A schematic structural diagram of a single-cell mass spectrometry analysis system based on parallel processing technology in an embodiment of the present invention is given, as shown in figure 1 As shown, the single-cell mass spectrometry system based on parallel processing technology includes:

[0032] Cell carrying unit 11, cleaning unit 13 and mass spectrometer 12, the mass spectrometer 12 has a sample inlet;

[0033] a base having a first axis of rotation;

[0034] A bracket 41, the bracket 41 is connected to the first rotating shaft and rotates around the first rotating shaft;

[0035] M groups of working units, M is an integer not less than 1, each group of working units includes P working units, P is an integer not less than 3; the working unit is arranged on the support, and the working unit includes a mechanical arm and Capillary needles, the capillary needles are arranged on the mechanical arm; in the rotation of the support 41, when the first work...

Embodiment 2

[0052] An application example of the single-cell mass spectrometry analysis system and method based on parallel processing technology according to Embodiment 1 of the present invention.

[0053] In this application example, if figure 1 As shown, a group of working units is set on the bracket 41, and the only group of working units includes three working units, and the three working units (the first working unit 21, the second working unit 22 and the third working unit 23) are uniformly Set on the entire circumference of the bracket 41, the central angle corresponding to the adjacent working unit is 120 degrees;

[0054] The cell carrying unit 11 is set at the first working position, the mass spectrometer 12 is set at the second working position, and the cleaning unit 13 is set at the third working position; the cell carrying unit 11 has cell carrying positions distributed in a matrix and isolated from each other 111 and a third container 112, the third container 112 is filled...

Embodiment 3

[0068] According to the application example of the single-cell mass spectrometry analysis system and method based on parallel processing technology in Embodiment 1 of the present invention, the difference from Embodiment 2 is:

[0069] like figure 2 As shown, there are two groups of work units, each group of work units includes three work units, the first group of work units includes the first work unit 21, the second work unit 22 and the third work unit 23, and the second group of work units includes the first work unit Four working units 27, the fifth working unit 28 and the sixth working unit 29, the six working units of the two groups of working units are evenly arranged on the entire circumference of the support 41, and the central angle corresponding to the two adjacent working units is 60 degrees ; Corresponding to the two groups of working units, the first cell carrying unit 113 and the second cell carrying unit 141 are in the first working position respectively, the ...

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Abstract

The invention provides a single-cell mass spectrometry system and method based on a parallel processing technology. The single-cell mass spectrometry system comprises: a cell bearing unit, a cleaningunit and a mass spectrometer; a base provided with a first rotating shaft; a bracket connected with the first rotating shaft and rotating around the first rotating shaft; and M groups of working units, wherein M is an integer not less than 1, each working unit comprises a mechanical arm and a capillary needle, the capillary needles are arranged on the mechanical arms, during the rotation of the bracket, when the first working unit in any group of working units is positioned at a first working position, the second working unit is positioned at a second working position, the third working unit is positioned at a third working position, the cell bearing unit is located at each first working position, the mass spectrometer is located at each second working position, and the cleaning unit is located at each third working position. The single-cell mass spectrometry system has the advantages of high working efficiency, accurate analysis result and the like.

Description

technical field [0001] The invention relates to cell analysis, in particular to a single-cell mass spectrometry analysis system and method based on parallel processing technology. Background technique [0002] Cells are the basic unit of the morphological structure and life activities of organisms. Life activities are realized in cells. The growth, differentiation, metabolism and reproduction of cells are all related to the overall state of cells. Most of the previous research is to analyze the population cells to get an average result. With the continuous improvement of analysis technology, it is found that there are differences between different individual cells. This difference between single cells is important in the life activities, physiological important in the study of pathological processes. However, the analysis of population cells cannot reflect the individual differences among cells and will cover up the information of individual cells. Therefore, single-cell an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N15/00G01N1/28
CPCG01N1/28G01N15/00G01N15/10G01N15/1031G01N2015/0065G01N2015/1006
Inventor 陈安琪闻路红李刚强甘剑勤陈腊胡舜迪洪欢欢
Owner NINGBO UNIV
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