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Odontobutis yaluensis sperm activating solution

A technology of snakehead and spermatozoa, applied in germ cells, microorganisms, biochemical equipment and methods, etc., can solve the problem of low success rate of artificial insemination, and achieve the purpose of improving activation rate, fertilization rate, and production efficiency. Effect

Pending Publication Date: 2021-03-19
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yalu sand snakehead is a different species from the northern snakehead. The northern snakehead sperm activation solution is not suitable for the duck green sand snakehead. So far, the problem of the low success rate of artificial insemination of the duck green sand snakehead has not been solved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] The sperm activating liquid of the duck green sand pond snakehead of the present invention is formed by mixing potassium chloride, sodium chloride and pure water, and contains potassium chloride 40mmol, sodium chloride 17mmol and water balance in every liter.

[0010] The preparation method is to put a certain amount of solute (potassium chloride, sodium chloride) into a beaker, add half the volume of the activation solution of pure water and stir to fully dissolve the solute in the beaker, then transfer the solution in the beaker into a volumetric flask, Wash the beaker three times with a small amount of pure water, pour the washed solution into a volumetric flask, and finally dilute to the required mark with pure water, and shake well.

Embodiment 2

[0012] The sperm activating liquid of the duck green sand pond snakehead of the present invention is formed by mixing potassium chloride, glycerin, Tris and pure water, and contains potassium chloride 40mmol, glycerin 33mmol, Tris 8mmol and water balance in every liter.

[0013] The preparation method is the same as in Example 1.

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PUM

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Abstract

The invention discloses an odontobutis yaluensis sperm activating solution. Each liter of the odontobutis yaluensis sperm activating solution contains 40 mmol of potassium chloride, 17 mmol of sodiumchloride and the balance of water, or 40 mmol of potassium chloride, 33 mmol of glycerol and 8 mmol of Tris are mixed with pure water, so that the activation rate of odontobutis yaluensis sperms can be effectively increased, and the rapid movement time and the service life can be effectively prolonged, wherein the highest activation rate is 95.00 + / -1.00%, the rapid movement time is 487.67 + / -15.82 s, and the service life is 1511.00 + / -105.83 s; and the fertilization rate of the odontobutis yaluensis can be effectively improved, so that the production efficiency of artificial propagation is improved.

Description

technical field [0001] The invention relates to the field of cultivation of channa chinensis, in particular to a sperm activation liquid of channa chinensis. Background technique [0002] Yalu sand pond snakehead ( Odontobutis yaluensis ), belonging to the order Perciformes, the suborder Gobies, the family Channaidae, and the genus Shatanga. It is a unique economic fish in the eastern mountainous area of ​​Liaoning. In recent years, due to overfishing and habitat changes, the wild resources of the ducklings have gradually decreased, and artificial breeding and breeding are needed to meet market demand. As one of the commonly used methods in production, dry insemination after artificial induced labor requires simple equipment and few restrictions, and can be used for large-scale artificial reproduction, and the fertilization rate is the most critical indicator of artificial reproduction production efficiency. Since the sperm in artificial insemination is inactive in the sem...

Claims

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Application Information

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IPC IPC(8): C12N5/076
CPCC12N5/061C12N2500/12C12N2500/35Y02A40/81
Inventor 骆小年李文康邓鸿圣段友健吴晨杨国军
Owner DALIAN OCEAN UNIV
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