High ammonia nitrogen tolerance improved variety breeding method for penaeus vannamei boone

A high ammonia nitrogen, tolerance technology, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of unstable traits, slow growth rate, low breeding rate of shrimp and shrimp fry, etc. Growth rate, improve the effect of stabilizing shrimp traits

Inactive Publication Date: 2021-04-02
海南禄泰海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially on the problem of high ammonia nitrogen tolerance of Penaeus vannamei, there has been a lack of a targeted breeding system, resulting in low breeding rate, slow growth rate and unstable traits of Penaeus vannamei

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for breeding fine varieties of Penaeus vannamei with high ammonia nitrogen tolerance, comprising the steps of:

[0023] (1) Broodstock breeding pond and water treatment for breeding: the selected area is 20m 2 The broodstock pond has a water depth of 1.2m, a shading net is set above the broodstock pond, and the light intensity is 60Lx; before using the broodstock pond, 80×10 -5 bleaching powder or 60×10 -6 Potassium permanganate disinfects broodstock ponds;

[0024] (2) Introduction: 12 strains of "Guangtai No. 1" and "Kehai No. 1" domestic self-selected varieties, natural populations in Central and South America, and the Gulf of Mexico were collected, independently raised, sampled, and tested by virus PCR. All negative strains were used as the basic population;

[0025] (3) Screening: Analyze the genetic diversity of the strains from each source, determine their genetic relationship, and keep 5 distant strains;

[0026] (4) Strengthening of parent-child sh...

Embodiment 2

[0038] A method for breeding fine varieties of Penaeus vannamei with high ammonia nitrogen tolerance, comprising the steps of:

[0039] (1) Broodstock breeding pond and water treatment for breeding: the selected area is 30m 2 The broodstock pond has a water depth of 1.4m, a shading net is set above the broodstock pond, and the light intensity is 80Lx; -5 bleach or 180×10 -6 Potassium permanganate disinfects broodstock ponds;

[0040] (2) Introduction: Collect 18 strains of domestic self-selected varieties "Guangtai No. 1", "Kehai No. 1", natural populations in Central and South America, and the Gulf of Mexico. All negative strains were used as the basic population;

[0041] (3) Screening: Analyze the genetic diversity of the strains from each source, determine their genetic relationship, and keep 8 distant strains;

[0042] (4) Strengthening of parent-child shrimp: raise to parent-child shrimp, select the ones with strong vitality, smooth body surface, no deformity, and bo...

Embodiment 3

[0054] A method for breeding fine varieties of Penaeus vannamei with high ammonia nitrogen tolerance, comprising the steps of:

[0055] (1) Broodstock breeding pond and water treatment for breeding: the selected area is 25m 2 The broodstock pond has a water depth of 1.3m, a shading net is set above the broodstock pond, and the light intensity is 70Lx; -5 bleach or 120×10 -6 Potassium permanganate disinfects broodstock ponds;

[0056] (2) Introduction: Collect 15 strains of domestic self-selected varieties "Guangtai No. 1", "Kehai No. 1", and natural populations in Central and South America, and the Gulf of Mexico. They are independently raised, sampled, and tested for virus PCR. All negative strains were used as the basic population;

[0057] (3) Screening: Analyze the genetic diversity of the strains from each source, determine their genetic relationship, and keep 6 distant strains;

[0058] (4) Strengthening of parent-child shrimp: raise to parent-child shrimp, select th...

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PUM

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Abstract

The invention relates to the technical field of prawn breeding, in particular to a high ammonia nitrogen tolerance improved variety breeding method for penaeus vannamei boone. The method comprises thefollowing steps of (1) parent shrimp culture pond and culture water treatment, (2) introduction, (3) screening, (4) parent shrimp strengthening, (5) family establishment, (6) seedling raising, (7) family independent culture, (8) seedling raising process management, (9) target family determination, (10) hybridization test, and (11) variety breeding. The method is suitable for a special high-ammonia-nitrogen culture system for penaeus vannamei boone, so that the breeding rate of penaeus vannamei boone seeds is increased, the growth speed is increased, and the traits of penaeus vannamei boone are stabilized.

Description

technical field [0001] The invention relates to the technical field of prawn breeding, in particular to a method for breeding fine varieties of Penaeus vannamei with high ammonia nitrogen tolerance. Background technique [0002] my country is a big country in Penaeus vannamei farming. It has become an important species of Penaeus vannamei in 1999. It has developed rapidly in the three southern provinces and has become the most popular shrimp species in the country. Serious problems such as uneven quality of seedlings, virus carrying and slow growth directly threaten the sustainable development of my country's vannamei aquaculture industry. Especially on the problem of high ammonia nitrogen tolerance of Penaeus vannamei, there has been a lack of a targeted breeding system, resulting in low breeding rate, slow growth rate and unstable traits of Penaeus vannamei. Therefore, we propose a breeding method for high ammonia nitrogen tolerance of Penaeus vannamei. Contents of the i...

Claims

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Application Information

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IPC IPC(8): A01K61/59
CPCA01K61/59Y02A40/81
Inventor 云永超云乐
Owner 海南禄泰海洋生物科技有限公司
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