High ammonia nitrogen tolerance improved variety breeding method for penaeus vannamei boone
A high ammonia nitrogen, tolerance technology, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of unstable traits, slow growth rate, low breeding rate of shrimp and shrimp fry, etc. Growth rate, improve the effect of stabilizing shrimp traits
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Embodiment 1
[0022] A method for breeding fine varieties of Penaeus vannamei with high ammonia nitrogen tolerance, comprising the steps of:
[0023] (1) Broodstock breeding pond and water treatment for breeding: the selected area is 20m 2 The broodstock pond has a water depth of 1.2m, a shading net is set above the broodstock pond, and the light intensity is 60Lx; before using the broodstock pond, 80×10 -5 bleaching powder or 60×10 -6 Potassium permanganate disinfects broodstock ponds;
[0024] (2) Introduction: 12 strains of "Guangtai No. 1" and "Kehai No. 1" domestic self-selected varieties, natural populations in Central and South America, and the Gulf of Mexico were collected, independently raised, sampled, and tested by virus PCR. All negative strains were used as the basic population;
[0025] (3) Screening: Analyze the genetic diversity of the strains from each source, determine their genetic relationship, and keep 5 distant strains;
[0026] (4) Strengthening of parent-child sh...
Embodiment 2
[0038] A method for breeding fine varieties of Penaeus vannamei with high ammonia nitrogen tolerance, comprising the steps of:
[0039] (1) Broodstock breeding pond and water treatment for breeding: the selected area is 30m 2 The broodstock pond has a water depth of 1.4m, a shading net is set above the broodstock pond, and the light intensity is 80Lx; -5 bleach or 180×10 -6 Potassium permanganate disinfects broodstock ponds;
[0040] (2) Introduction: Collect 18 strains of domestic self-selected varieties "Guangtai No. 1", "Kehai No. 1", natural populations in Central and South America, and the Gulf of Mexico. All negative strains were used as the basic population;
[0041] (3) Screening: Analyze the genetic diversity of the strains from each source, determine their genetic relationship, and keep 8 distant strains;
[0042] (4) Strengthening of parent-child shrimp: raise to parent-child shrimp, select the ones with strong vitality, smooth body surface, no deformity, and bo...
Embodiment 3
[0054] A method for breeding fine varieties of Penaeus vannamei with high ammonia nitrogen tolerance, comprising the steps of:
[0055] (1) Broodstock breeding pond and water treatment for breeding: the selected area is 25m 2 The broodstock pond has a water depth of 1.3m, a shading net is set above the broodstock pond, and the light intensity is 70Lx; -5 bleach or 120×10 -6 Potassium permanganate disinfects broodstock ponds;
[0056] (2) Introduction: Collect 15 strains of domestic self-selected varieties "Guangtai No. 1", "Kehai No. 1", and natural populations in Central and South America, and the Gulf of Mexico. They are independently raised, sampled, and tested for virus PCR. All negative strains were used as the basic population;
[0057] (3) Screening: Analyze the genetic diversity of the strains from each source, determine their genetic relationship, and keep 6 distant strains;
[0058] (4) Strengthening of parent-child shrimp: raise to parent-child shrimp, select th...
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