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Application of traditional Chinese medicine toosendanin in preparation of product for preventing and treating glioma of nervous system

A nervous system and toosendanin technology, applied in the field of medicine, can solve the problems that the average survival rate is less than 1 year, the prognosis of patients with nervous system glioma is poor, and the survival rate is less than 5%.

Inactive Publication Date: 2021-04-06
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, despite advances in treatment strategies, the prognosis of patients with neuroglioma remains poor, with an average survival rate of less than 1 year and a two-year survival rate of less than 5%.

Method used

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  • Application of traditional Chinese medicine toosendanin in preparation of product for preventing and treating glioma of nervous system
  • Application of traditional Chinese medicine toosendanin in preparation of product for preventing and treating glioma of nervous system
  • Application of traditional Chinese medicine toosendanin in preparation of product for preventing and treating glioma of nervous system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Effects of Chuanxiong on Proliferation of Glioma Cells:

[0031]Cell culture: U87 glioma cell line is used with a DMEM medium containing 10% fetal bovine serum, 1% penicillin, 1% streptomycin, placed at 37 ° C, 5% CO2Culture in the incubator and liquidation over the same day.

[0032]Reagent formulation: Chuanximin (TSN) is configured to 2, 5, 10, 20, 40, 60, 80, 100 nmol / L8 different concentrations of solutions by fully medium.

[0033]Proliferation inhibition: Collect the logarithmic growth cell and adjust the concentration of the cell suspension, take 5 96-well plates, add 100 μl of cell suspension per well, and the number of cells to be tested is 1 × 105 / Hole (edge ​​hole with sterile PBS to eliminate edge effect), 37 ° C, 5% CO2After 24 hours, the culture solution was taken out, and 8 different concentrations of TSN solution preparation were added to the reagent, and 6, 12, 24, 36, 48 hours were added, and a 96-well plate was taken out, and 5 mg / ml CCK-8 was added each other,...

Embodiment 2

[0036]Effects of Chuanxiong on the level of STAT3 phosphorylation level in glioma cells:

[0037]Cell culture: U87 glioma cell line is used with a DMEM medium containing 10% fetal bovine serum, 1% penicillin, 1% streptomycin, placed at 37 ° C, 5% CO2Culture in the incubator and liquidation over the same day.

[0038]Reagent formulation: Chuanximin (TSN) is configured to 0, 10, 20, 40 nmol / L 4 different concentrations of TSN solutions by fully medium.

[0039]Cell vaccination and administration: Collecting a total growth cell and adjusting the cell suspension concentration, take 3 6-well plate, add 3000 μL of cell suspension per well, and the plate is 1 × 106 / Hole, 37 ° C, 5% CO2After 24 hours, 4 different concentrations of TSN solutions formulated separately were added, and the culture was continued for 24 hours, and the 6-well plate was taken out, and the cells were collected. The Western Blot method detects the amount of STAT3 protein.

[0040]Experimental results:figure 2 As shown, STAT3 ...

Embodiment 3

[0042]Effect of TSN on tumor morphology of nude mice parallel glue model

[0043]Animal model was established: a three-week old female BALB / C nude mice, feeding under SPF-level sterile conditions, controlled room temperature during the experiment (20 ± 2) ° C, humidity 48%, 12 hours alternating lighting. The U87 glioma cells were collected, and 1000R / min centrifuge was centrifuged for 5 min, and the supernatant was removed, and the cells were suspended in a serum-free DMEM medium solution, adjusted the cell concentration of 2.5 × 10.7 / ml. Take the cell suspension inoculated in the right rib in the mouse, the number of cells was 5 × 106A / only, the inoculation volume was 0.2 ml / only, and the nude mice subside the tumor-tumor model was established.

[0044]Reagent formulation: Chuanximin (TSN) was prepared from 0.5% carboxymethylcellulose sodium to 0.06, 0.24 mg / ml3 different concentrations of TSN solutions.

[0045]Animal grouping and administration: mice established a model were ra...

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Abstract

The invention relates to the technical field of medicine, in particular to application of traditional Chinese medicine toosendanin in preparation of products for preventing and treating glioma of the nervous system. Glioma of the nervous system is a tumor which is highly harmful to a patient in intracranial tumors, due to the existence of a blood brain barrier, the treatment effect of traditional chemotherapeutic drugs on the glioma of the nervous system is not ideal, and the main treatment means is mainly operation. Furthermore, a chemical entity with an inhibiting effect on the glioma of the nervous system is developed, so that the operation proportion can be well reduced, and the pain of a patient in treatment is reduced. In-vitro experiments find that toosendanin can inhibit proliferation of glioma cell strains of the nervous system and induce apoptosis, and in-vivo experimental studies find that toosendanin can inhibit growth of glioma of the nervous system and prolong the survival time of tumor-bearing nude mice. As toosendanin has the characteristics of low toxicity and environment friendliness, toosendanin can be used for preparing the nervous system glioma treatment medicine.

Description

Technical field[0001]The present invention relates to the field of medical technology, and more particularly to the application of Chinese drugs in the preparation of glioma products for preparation prevention and treatment of nervous system.Background technique[0002]Grid is the most common malignant tumor in the central nervous system, 80% of malignant glioma, which is the highest type of glioma. As the course of the disease, the patient's cognitive function will affect, and it is easy to relapse, and the patient's quality of life is seriously affected.[0003]At present, the main treatment for neurological glioma is surgical treatment, followed by radiation therapy and chemical drug treatment. However, although the treatment strategy has progressed, the prognosis of patients with neurological glioma is still in good, and the average survival rate is still less than 1 year, and the two-year survival rate is less than 5%. Therefore, a means of more effective treatment is required to c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61P35/00A61P25/00A23L33/105
CPCA61K31/58A61P35/00A61P25/00A23L33/105A23V2002/00A23V2200/308A23V2200/322A23V2250/21
Inventor 杨乐徐媛招明高
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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