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Calibration bin, calibration system and method for sampling physical efficiency of air microorganism sampler

An air microorganism and sampler technology, applied in the field of measurement, to achieve the effect of ensuring accuracy, convenient operation and movement, and simple operation

Active Publication Date: 2021-04-06
NAT INST OF METROLOGY CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no universal air microbial sampler sampling physical efficiency measurement and calibration device in China. In order to improve and ensure the accuracy of air microbial sampler sampling results and ensure the comparability of test results of air microbial samplers from various manufacturers, it is necessary to develop air The sampling physical efficiency calibration system of microbial samplers realizes the calibration of instruments and ensures the traceability and reliability of the measurement results of air microbial samplers in my country

Method used

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  • Calibration bin, calibration system and method for sampling physical efficiency of air microorganism sampler
  • Calibration bin, calibration system and method for sampling physical efficiency of air microorganism sampler
  • Calibration bin, calibration system and method for sampling physical efficiency of air microorganism sampler

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Effect test

Embodiment 1

[0044] Air microbial sampler sampling physical efficiency calibration chamber ( figure 2 ) including clean air system 2, aerosol chamber 1 ( image 3 ), spoiler fan unit 3, exhaust gas treatment module 7, negative pressure regulation module 4, ambient temperature and humidity regulation module 5, environmental parameter monitoring module 6, cleaning, disinfection and sterilization module 8, and host computer monitoring module 9;

[0045] The upstream of the aerosol chamber 1 is connected to the clean air system 2 to ensure the cleanliness of the air entering the aerosol chamber 1 . At the same time, the downstream of the aerosol chamber 1 is connected with the exhaust gas treatment module 7 to ensure the compliance of the exhaust gas in the experiment. The aerosol chamber 1 has various auxiliary modules, including an environment temperature and humidity adjustment module, an environment parameter monitoring module 6, a negative pressure adjustment module, a spoiler fan unit ...

Embodiment 2

[0059] An air microbial sampler sampling physical efficiency calibration system containing an air microbial sampler sampling physical efficiency calibration chamber, and a monodisperse aerosol generator 24 (this generator is used to generate monodisperse particles with a particle size of 0.7 μm to 15 μm); aerosol generation Interface 25, which is used to introduce the aerosol produced by the above-mentioned monodisperse aerosol generator 24 into the above-mentioned calibration chamber; 2 air particle counters, which measure the measured sampler at the same time and the membrane filtration sampler at the same time. Concentration of particles of different particle sizes; calibration standard substance solution, used to form aerosols containing biological particles of different particle sizes, preferably, said calibration standard substance solution is potassium iodide ethanol of different concentrations containing Bacillus atrophaeus standard substance solution; also includes an ...

Embodiment 3

[0063] The specific operation steps for calibrating the sampling physical efficiency of the air microbial sampler by using the air microbial sampler sampling physical efficiency calibration system of embodiment two are as follows:

[0064] Step 1: Preparation of Standard Strain Suspension

[0065] (1) take (10 6 ~10 7 ) CFU / mL of Bacillus atrophaeus spore suspension 1mL, respectively placed in five sterilized centrifuge tubes for centrifugation, discarding the supernatant to obtain the spores;

[0066] (2) be respectively dispersed in the 80% ethanol solution that the mass concentration of potassium iodide of 9mL is respectively 0%, 0.007%, 0.07%, 0.7% and 7%, the spore concentration that prepares is 10% 5 5 suspensions in CFU / mL;

[0067] Step 2: Prepare solid medium

[0068] (1) Weigh 40.0 g of Tryptone Soy Agar (TSA), dissolve it in 1000 mL of distilled water, and distribute it in conical flasks;

[0069] (2) Autoclave at 121°C for 15 minutes. When the temperature drop...

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Abstract

The invention discloses a calibration bin, calibration system and method for the sampling physical efficiency of an air microorganism sampler. The calibration bin comprises an aerosol chamber, a clean air system, an environment temperature and humidity adjustment module, an environment parameter monitoring module, a negative pressure adjustment module, a turbulent flow fan set, a cleaning, disinfection and sterilization module, a waste gas treatment module, an upper computer monitoring module, an an aerosol generating interface. The calibration system comprises the calibration bin for the sampling physical efficiency of the air microorganism sampler, a monodisperse aerosol generator, an air particulate matter particle counter, a membrane filtration air microorganism sampler comparator and a potassium iodide ethanol solution containing bacillus atrophaeus standard substances for calibration. According to the invention, the problem that no calibration device is provided for the sampling physical efficiency of the existing air microorganism sampler is solved, and the calibration of the sampling physical efficiency of five bacteria-containing particles with the particle size of 0.7-15 [mu]m generated by the monodisperse aerosol generator can be realized.

Description

technical field [0001] The present invention relates to the field of measurement, in particular to the technical field of sampling physical efficiency of an air microbial sampler, and more specifically, to a calibration device, system and method for sampling physical efficiency of an air microbial sampler. Background technique [0002] The air microbial sampler is a sampling instrument specially used to collect microorganisms in the air. Based on the Anderson sampling principle, the sampler extracts air according to a certain flow rate, so that the microbial particles in the airflow accelerate and hit the surface of the nutrient agar culture dish, which can be seen by the naked eye after cultivation. Count the number of colonies and calculate the concentration of microorganisms in the air according to the sampling volume. [0003] Air microbial sampler can be used to monitor microbial indicators in ordinary air environment and clean air environment. The air microbial sample...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/38C12M1/36C12M1/26C12M1/12C12M1/04C12M1/00C12Q3/00C12Q1/24
CPCC12M41/36C12M41/12C12M41/34C12M41/40C12M41/48C12M33/14C12M33/00C12M37/00C12M29/20C12Q3/00C12Q1/24
Inventor 傅博强刘玉杰隋志伟李曼莉唐治玉李浩高颖王晶戴新华郑劲林
Owner NAT INST OF METROLOGY CHINA
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