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Breeding process of transgenic bollworm resisting cotton hybrid

A technology of hybrid species and cotton bollworm, applied in the direction of plant genetic improvement, botanical equipment and methods, application, etc., can solve the problems of farmland ecological environment pollution, increase the cost of cotton planting, poor chemical control effect, etc., and achieve improvement of agronomic traits, The effect of improving breeding efficiency and shortening the number of years

Inactive Publication Date: 2003-11-05
NANJING AGRICULTURAL UNIVERSITY
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  • Description
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AI Technical Summary

Problems solved by technology

Chemical control less effective as bollworm resistance grows
Extensive use of chemical pesticides to prevent and control cotton bollworm not only increases the cost of cotton planting, increases the labor intensity of cotton farmers, but also causes pollution to the farmland ecological environment

Method used

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  • Breeding process of transgenic bollworm resisting cotton hybrid
  • Breeding process of transgenic bollworm resisting cotton hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] The original basic material "SGK321" of the Bt+CpTI gene insect-resistant cotton issued by the Biotechnology Center of the Chinese Academy of Agricultural Sciences utilized by the present invention has general economic properties (only about 70 kilograms per mu), and insect resistance exists between strains (purity is about 95%). For this purpose, individual plant selection and progeny identification were carried out on this material. Individual plants and strains homozygous for resistance to cotton bollworm were selected, and various methods were adopted for resistance identification.

[0028] Kanamycin marker identification: Tear the absorbent cotton into small strips, dip it in 500ppm kanamycin solution, and stick it on the new leaves of the cotton plant. After 5 days, the leaves of all susceptible cotton plants stained with kanamycin solution showed obvious yellow spots, while the leaves of Bt+CpTI gene-transferred insect-resistant cotton had no symptoms.

[0029]...

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Abstract

The invention discloses a method for breeding transgenic cotton bollworm-resistant cotton hybrids, which is specially used for the breeding of cotton bollworm-resistant cotton hybrids transgenic with Bt+CpTI bivalent genes. The insect resistance identification and breeding of the transgenic insect-resistant cotton "SGK321" were carried out, and the insect-resistant homozygous (100%) transgenic insect-resistant cotton "SGK321'" with lint yield increased by 9.5% compared with the raw material was obtained. The insect-resistant hybrid "Nankang 7" was bred with "SGK321'" as the male parent and "Sumian 12" as the female parent. The hybrid has high yield (90-150 kg of lint per mu), high resistance to cotton bollworm and can restrain possible resistance of cotton bollworm to cotton.

Description

Field of invention: [0001] The invention discloses a method for breeding transgenic cotton bollworm-resistant cotton hybrids, which belongs to cotton transgenic breeding methods and is specially used for the breeding of cotton bollworm-resistant cotton hybrids transgenic with Bt+CpTI bivalent genes. technical background: [0002] Cotton bollworm is the main pest that harms cotton, and it has caused great losses to cotton production in my country. With the enhancement of the resistance of cotton bollworm, the effect of chemical control is poor. Extensive use of chemical pesticides to control cotton bollworm not only increases the cost of cotton planting, increases the labor intensity of cotton farmers, but also causes pollution to the farmland ecological environment. Breeding insect-resistant cotton varieties is an important measure for the integrated control of cotton bollworm. The Bt+CpTI bivalent gene insect-resistant hybrid cotton has significant resistance to cotton bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00A01H1/02
Inventor 张天真朱协飞唐灿明郭旺珍
Owner NANJING AGRICULTURAL UNIVERSITY
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