Cow milk and goat milk adulteration identification method based on phosphatidylcholine

Abstract

The invention discloses a cow milk and goat milk adulteration identification method based on phosphatidylcholine, and belongs to the technical field of dairy product detection. The method comprises the following steps: separating and extracting lipid components from a milk sample by adopting a trichloromethane-methanol mixed solution; carrying out column separation and purification on the lipid components obtained by adopting liquid chromatography, and carrying out information acquisition on the purified lipid components by adopting a high-throughput mass spectrometry technology to obtain a peak table of the lipid components; carrying out statistical difference analysis and stoichiometric analysis on information in the peak table of the obtained lipid components to obtain differential phosphatidylcholine used for adulteration of goat milk as a marker; and carrying out quantitative analysis on the obtained differential phosphatidylcholine for adulteration of the goat milk, and detecting a sample to be identified to realize adulteration identification of the cow milk and the goat milk. The method has high repeatability and sensitivity, can be used for rapid analysis of small samples, and is helpful for better and faster adulteration identification of cow milk and goat milk samples.

Description

technical field [0001] The invention belongs to the technical field of dairy product detection, and relates to a phosphatidylcholine-based method for identifying bovine and goat milk adulteration. Background technique [0002] Dairy products are the main source of lipids in people's daily diet. Due to the important role of dietary lipids in the pathogenesis of diseases, the lipid composition of milk and dairy products has been systematically studied, especially the fatty acid composition and total fat content. The content of saturated fatty acids in dairy products has an important impact on health. Eating habits high in saturated fatty acids are related to the occurrence of metabolic diseases. Therefore, researchers are constantly trying to reduce the content of saturated fatty acids in dairy products and their products. There are species differences in the lipid composition of dairy products, with small ruminant milk such as goat's milk having a higher concentration of med...

AI Technical Summary

Problems solved by technology

[0003] The current methods for identifying adulterated cow and goat milk are mainly based on fluorescence spectroscopy to detect small molecule metabolites such as vitamins and amino acids, and to judge their composition differences in cow and goat milk, but this analysis method has low sensitivity and repeatability; based on Chromatography-mass spectrometry is used to detect the fatty acid profile and determine its composition difference in cow and goat milk. The pretreatment is complicated and derivatization such as heating is required; Milk samples, whey or milk fat globule membrane protein macromolecule composition differences are detected, but the protein detection and analysis takes a long time, the biological reagents used are highly toxic, and more raw materials are required

Therefore, at present, there is no quick, easy, and highly reproducible method for the identification of bovine and goat milk adulteration.

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