Codonopsis Wall. species cpSSR molecular marker and application thereof

A technology of molecular markers and species, applied in the field of plant molecular biology, can solve problems such as lack

Active Publication Date: 2021-06-04
SHANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of Codonopsis species cpSSR molecular marker and its application for the lack of existing Codonopsis species cytoplasmic markers

Method used

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  • Codonopsis Wall. species cpSSR molecular marker and application thereof
  • Codonopsis Wall. species cpSSR molecular marker and application thereof
  • Codonopsis Wall. species cpSSR molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Analysis of cpSSR loci in Codonopsis pilosula

[0042] Use MISA software to search for SSR sites in the chloroplast genomes of Codonopsis pilosula and Codonopsis qinlings. The software setting standards are: base pair, bp 1, 2, 3, 4, 5, 6 bp, and the number of repetitions is not less than 10, 6, 5, 5, 5, 5, 5 times, the composite SSR is less than 100bp. The results showed that there were 29 cpSSR loci in the chloroplast genome of Codonopsis verticillum, among which there were 25 mononucleotide repeat motif A / T loci, accounting for 86.20% of the total loci; dinucleotide repeat motif ( AT / AT) and trinucleotide repeat motif (AAG / CTT) each have two cpSSR sites, accounting for 6.9% of the total number of cpSSR sites (Table 1).

[0043] Table 1 Different SSR repeat motifs in Codonopsis pilosula

[0044]

[0045] Codonopsis cpSSR primer design

[0046] Use primer 5.0 for cpSSR marker design, combined with manual adjustment of primers. The design criteria of the primers ...

Embodiment 2

[0050] Utilize five pairs of primers of the cpSSR molecular marker provided by the present invention to carry out rapid identification of Codonopsis genus species, comprising the following steps:

[0051] (1) Material collection and DNA extraction

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Abstract

The invention relates to the technical field of plant molecular biology, in particular to a Codonopsis Wall. species cpSSR molecular marker and application thereof. According to the content of the Codonopsis Wall. species cpSSR molecular marker and the application thereof, five pairs of specific cpSSR markers are designed and developed on the basis of analysis of chloroplast genome sequences of Codonopsis Wall. plants, and the markers are disclosed to be capable of rapidly identifying codonopsis lanceolata species; and as different Codonopsis Wall. species are difficult to accurately distinguish in plant, fruit, seed and root forms, the developed cpSSR molecular marker can be used for quickly and accurately identifying a plurality of Codonopsis Wall. species. The Codonopsis Wall. species cpSSR molecular marker has the advantages of high development efficiency, low period and low cost, and fills the blank of no cytoplasm genome molecular marker in codonopsis at present.

Description

technical field [0001] The invention relates to the technical field of plant molecular biology, in particular to a cpSSR molecular marker of Codonopsis genus species and its application. Background technique [0002] Codonopsis Wall. is a perennial herbaceous plant belonging to the family Campanulaceae, Codonopsis Wall. There are 39 species in my country and the roots of most species have medicinal value. In the current clinical application, all kinds of Codonopsis ginseng, pearl ginseng, egg ginseng, etc. have different degrees of effects such as nourishing the spleen, promoting body fluid, promoting lactation, eliminating phlegm, relieving cough, stopping bleeding, replenishing qi, and solidifying. , Shrink the uterus, inhibit tachycardia and other effects. Codonopsis pilosula is currently the most widely used in this genus. It has been widely used and has become a bulk medicinal product, including Sichuan Codonopsis pilosula, Guanhua Codonopsis pilosula, Gray Hairy Codon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 詹海仙
Owner SHANXI UNIV OF CHINESE MEDICINE
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