Molecular identification method of medicinal materials Microctis Foliumand Microcos chungii

A technology of distributing dregs and rags, which is applied in the fields of biotechnology and molecular biology, can solve the problems of unseen molecular identification research, undiscovered HRM combined with DNA barcode identification method, etc., to achieve rapid identification and separation, and applicability Broad, stable identification of effects

Active Publication Date: 2021-06-25
GUANGZHOU WANGLAOJI MAJOR HEALTH IND +1
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no other molecular identification studies using this method for the identification of medicinal material cloth residue leaves and their counterfeit products, and so far there has not been any report on the identification method of HRM combined with DNA barcodes for medicinal materials cloth residue leaves and Hainan rag leaves

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular identification method of medicinal materials Microctis Foliumand Microcos chungii
  • Molecular identification method of medicinal materials Microctis Foliumand Microcos chungii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The basic plant identification of embodiment 1 medicinal material cloth dregs leaf and Hainan rags leaf

[0045] Sample acquisition: A total of 13 samples of the original plants and medicinal materials of Buzayeji from different origins were collected, and a total of 6 samples of the original plants of Buzayeji in Hainan (see Table 1 for details).

[0046] serial number sample number sample name Sampling site sample source 1 BZY1 slag leaves b

blade Hebei Anguo 2 BYZGR Cloth leaves b

blade Hebei Anguo 3 BZY3 Cloth leaves b

blade Anhui Bozhou 4 BYZGR4 Cloth leaves b

blade Anhui Bozhou 5 BZY5 slag leaves b

blade Jiangxi camphor tree 6 BZY6 slag leaves b

blade Jiangxi camphor tree 7 TH10 slag leaves a

blade Hainan Wanning 8 TH11 slag leaves a

blade Yangjiang, Guangdong 9 TH12 slag leaves a

blade Maoming, Guangdong ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biotechnology and molecular biology, and particularly relates to a molecular identification method of medicinal materials Microctis Folium and Microcos chungii. The molecular identification method provided by the invention mainly comprises the following steps of: extracting whole genome DNA from a sample; taking the DNA sample as a template, amplifying a fragment containing an ITS2 sequence, and carrying out HRM-PCR analysis on the fragment; and distinguishing the medicinal materials Microctis Folium and Microcos chungii according to the difference of an ITS2-HRM curve. The molecular identification method provided by the invention is simple to operate, and realizes accurate, efficient and rapid identification of the medicinal material Microctis Folium and the adulterant Microcos chungii.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and molecular biology, and particularly relates to a molecular identification method for medicinal material cloth slag leaves and Hainan rag leaves. Background technique [0002] The medicinal material of cloth slag leaves is the dried leaves of the tilia family Microcos paniculata L., also known as sage leaves, rag leaves, linen leaves, etc. Mainly produced in Guangdong, Guangxi, Hainan, Yunnan and other provinces. The 2020 edition of the "Chinese Pharmacopoeia" records that the leaves are slightly acidic and cool. Return to the spleen and stomach meridians. It has the effect of digestion and stagnation, clearing away heat and removing dampness. For food stagnation, cold and fever, damp-heat jaundice. Modern research has shown that slag leaves are rich in a variety of flavonoid active ingredients such as isorhamnetin, kaempferol, and quercetin. It has the functions of antipyretic, anal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/172C12Q2527/107
Inventor 陈士林孙伟孟祥霄熊超李泽鑫师玉华徐文流翁少全郑荣波李词周张丰源王艳芳
Owner GUANGZHOU WANGLAOJI MAJOR HEALTH IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap