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Application of cucumber glutaredoxin gene csgrx4 and its susceptibility to Botrytis cinerea

A technology for botrytis cinerea and disease susceptibility, applied in the application field of cucumber glutaredoxin gene CsGRX4 and its susceptibility to Botrytis cinerea, to achieve the effect of improving disease susceptibility

Active Publication Date: 2022-07-26
NORTHWEST A & F UNIV
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Problems solved by technology

[0006] It has been known that glutaredoxin genes in other plants are involved in the regulation of Botrytis cinerea fungal diseases, but the involvement of glutaredoxin genes in cucumber in response to Botrytis cinerea fungal diseases and its function have not been reported.

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  • Application of cucumber glutaredoxin gene csgrx4 and its susceptibility to Botrytis cinerea
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  • Application of cucumber glutaredoxin gene csgrx4 and its susceptibility to Botrytis cinerea

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[0018] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0019] The cucumber glutaredoxin gene CsGRX4 increases the susceptibility of the pathogenic fungus Botrytis cinerea in transgenic plants (Arabidopsis thaliana).

[0020] According to the homologous recombination technology, the present invention refers to the genome sequence of North China type cucumber 9930, takes the first strand of cDNA synthesized by reverse transcription of the total RNA of cucumber leaves as a template, and amplifies glutaredoxin for the first time through polymerase chain reaction technology. The gene CsGRX4. The sequence of the coding region of the gene is 339bp, ...

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Abstract

The invention relates to the technical field of plant genetic engineering, in particular to the application of a cucumber glutaredoxin gene CsGRX4 and its botrytis susceptibility. The invention finds that a cucumber glutaredoxin gene CsGRX4 is infected with the corresponding pathogenic fungus Botrytis cinerea, and its overexpression can obviously improve the susceptibility to the pathogenic fungus Botrytis cinerea. After quantitative inoculation of Botrytis cinerea spore suspension, the lesion area of ​​the transgenic lines overexpressing CsGRX4 was significantly larger than that of the wild-type control. The above results indicated that the overexpression of cucumber glutaredoxin gene CsGRX4 in Arabidopsis increased the transgenic Arabidopsis) susceptibility to the pathogenic fungus Botrytis cinerea.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the application of a cucumber glutaredoxin gene CsGRX4 and its botrytis susceptibility. Background technique [0002] In vegetable production, plant fungal diseases have always been an important factor limiting the improvement of vegetable yield and quality. The response of vegetable crops to fungal infection can be divided into disease resistance or susceptibility. Some key genes or transcription factors play a very important role in pathogen infection and defense responses against pathogens. When plants are susceptible to disease, pathogenic bacteria induce up-regulation of the expression of some key genes or inhibit the defense response of plants through infection, including the regulation of signaling pathways closely related to disease defense responses and the regulation of disease resistance gene expression, thereby causing the occurrence of diseases. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N15/82A01H5/00A01H6/20
CPCC12N9/0004C12N15/8282C12Y120/04001
Inventor 陈书霞杨玉婷
Owner NORTHWEST A & F UNIV
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