Application of DNA tetrahedron in preparation of medicine for preventing and treating type 1 diabetes mellitus

A technology of type 1 diabetes and tetrahedron, applied in drug combination, metabolic disease, pharmaceutical formula, etc., can solve the problem of tFNAs treatment or prevention of type 1 diabetes that has not yet been seen, and achieve the effect of solving insufficient insulin secretion

Active Publication Date: 2021-08-06
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] There are no reports on the treatment

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  • Application of DNA tetrahedron in preparation of medicine for preventing and treating type 1 diabetes mellitus
  • Application of DNA tetrahedron in preparation of medicine for preventing and treating type 1 diabetes mellitus
  • Application of DNA tetrahedron in preparation of medicine for preventing and treating type 1 diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The synthesis of embodiment 1tFNAs

[0038] 1. Synthesis method

[0039] Dissolve the four DNA single strands (S1, S2, S3, S4) in TM Buffer (10mM Tris-HCl, 50mM MgCl 2 , pH = 8.0), so that the final concentration of the four DNA single strands is 1000nM, mix well, heat quickly to 95°C and keep for 10 minutes, then quickly cool down to 4°C and keep for more than 20 minutes to obtain tFNAs. Synthesis schematic as figure 1 As shown in A.

[0040] The sequences of the four single strands (5'→3') are as follows:

[0041] S1:

[0042] ATTTATCACCCGCCATAGTAGACGTATCACCAGGCAGTTGAGACGAACATTCCTAAGTCTGAA (SEQ ID NO. 1)

[0043] S2:

[0044] ACATGCGAGGGTCCAATACCGACGATTACAGCTTGCTACACGATTC AGACTTAGGAATGTTCG (SEQ ID NO. 2)

[0045] S3:

[0046] ACTACTATGGCGGGTGATAAAACGTGTAGCAAGCTGTAATCGACGGGAAGAGCATGCCCATCC (SEQ ID NO. 3)

[0047] S4:

[0048] ACGGTATTGGACCCTCGCATGACTCAACTGCCTGGTGATACGAGGATGGGCATGCTCTTCCCG (SEQ ID NO. 4)

[0049] 2. Identification

[0050] From the results o...

experiment example 1t

[0052] Experimental example 1 In vivo experiment of tFNAs preventing diabetes

[0053] NOD / ShiLtJ mice are a typical animal model for simulating type 1 diabetes. At week 4-5, lymphocytes appear around the islets, and then a large number of white blood cells enter the entire islets. Finally, the β-cells fail, insulin deficiency begins, and symptoms of diabetes appear. In females, pancreatic insulin drops significantly at about 12 weeks (male is a few weeks later than females), and blood sugar levels rise to greater than 2.50 mg / mL; 90% of pancreatic islet β cells are destroyed at 24-30 weeks, and the probability of females developing diabetes is 90% , males have a 50%-60% chance of developing diabetes.

[0054] In this experiment, female NOD / ShiLtJ mice were treated with tFNAs to evaluate the effect of tFNAs on preventing and inhibiting the progression of diabetes.

[0055] 1. Method

[0056] (1) 8-week-old female NOD / ShiLtJ mice were purchased and adaptively fed for one week. ...

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PUM

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Abstract

The invention discloses application of a DNA tetrahedron in preparation of a medicine for preventing and treating type 1 diabetes mellitus, and belongs to the field of diabetes mellitus medicines. According to the application, a regulating effect of the DNA tetrahedron on immune cells is utilized, autoimmunity is inhibited, and occurrence and development process of type 1 diabetes mellitus is hindered from the source; in addition, the DNA tetrahedron and metabolites thereof are non-toxic and good in biocompatibility, so that the DNA tetrahedron has a good application prospect when being used for preparing the medicine for preventing and treating the type 1 diabetes mellitus.

Description

technical field [0001] The invention belongs to the field of diabetes-related drugs. Background technique [0002] Type 1 diabetes is the most common autoimmune disease. As an autoimmune disease, the immune pathogenesis of type 1 diabetes is mainly T cell-mediated attack on the insulin-secreting β cells in the islets. With the progress of this autoimmune process, the number of pancreatic β cells gradually decreases, and eventually Lead to severe lack of insulin secretion, resulting in glucose metabolism dysfunction. [0003] At present, the most important treatment method for type 1 diabetes is subcutaneous injection of insulin, but this method often makes the patient's blood sugar fluctuate greatly, easily causing hypoglycemia syncope; and injection of insulin can only temporarily control the condition, and cannot cure type 1 diabetes. [0004] Pancreatic islet transplantation can help patients produce their own insulin, but clinical practice shows that patients do not re...

Claims

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Application Information

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IPC IPC(8): A61K31/7088A61P3/10
CPCA61K31/7088A61P3/10
Inventor 林云锋高邵静雅蔡潇潇
Owner SICHUAN UNIV
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