Composition for microscopic observation as well as preparation method and application thereof

A composition and microscope technology, which is applied in the preparation, sampling, and instrumentation of test samples, can solve problems such as toxicity and high price, and achieve the effect of accurate differential diagnosis and clear imaging.

Pending Publication Date: 2021-08-06
吴敏 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used dehydrating agents and clearing agents are compounds such as absolute ethanol and chloroform, which are expensive and some have certain toxicity
In addition, the current preparation of specimens of cells and microorganisms is usually given a given ratio, and only one final state is produced. There is a lack of gradual research and detailed analysis of the entire process, so many phenomena are only half-understood.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Accurately weigh 8g of sodium chloride and 0.2g of ammonium oxalate to prepare solutions with the following concentrations: 1%, 5%, 10%, 15%, 20%, and 25%.

[0029] When in use, the Saccharomyces cerevisiae cell samples to be observed are processed sequentially in the order of concentration from low to high, and the samples to be observed are made into slide specimens for observation with an optical microscope, which has a good imaging effect.

Embodiment 2

[0031] Accurately weigh 8g of sodium chloride, 0.2g of ammonium oxalate, and 0.005g of sodium glutamate, and prepare solutions with the following concentrations: 1%, 5%, 10%, 15%, 20%, and 25%.

[0032] When in use, the Saccharomyces cerevisiae cell samples to be observed were processed sequentially in the order of concentration from low to high, and the samples to be observed were made into slide specimens, observed with an optical microscope, and the imaging effect was found to be better than that of Example 1.

Embodiment 3

[0034] Accurately weigh 8.5g of sodium chloride, 0.5g of ammonium oxalate, and 0.001g of sodium glutamate, and prepare solutions with the following concentrations: 5%, 10%, 15%, 20%, 25%, 30%, and 35%.

[0035] When in use, the Bacillus subtilis cell samples to be observed are processed sequentially in the order of concentration from low to high, and, after each cell sample is treated with a solution of a new concentration, the sample to be observed is made into a slide specimen, using The optical microscope is used for observation, thus realizing precise observation of the dynamic change process of the cell sample after treatment, with excellent imaging effect.

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PUM

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Abstract

The invention relates to a composition for microscopic observation as well as a preparation method and application thereof, and the composition comprises the following components in parts by weight: 80 to 100 parts of sodium chloride and 1 to 5 parts of ammonium oxalate, and further comprises 0.01 to 0.2 part of sodium glutamate. The invention further provides a corresponding solution and the composition, when the solution and the composition are used for preparing a cell sample observed under a microscope, a concentration gradient treatment mode is adopted, so that most of cell and microorganism samples can keep original forms, the solution and the composition are suitable for sample observation in a larger range, and the solution and the composition have the advantages of clear imaging, low cost, easiness in operation and the like.

Description

technical field [0001] The invention relates to the field of biological sample preparation, in particular to a composition for microscope observation and its preparation method and application. Background technique [0002] In the living research of cells and microbiological tissues, it is often necessary to use dehydrating agents and transparent agents to make biological specimens, so as to observe the internal structure of cells and microorganisms through a microscope, and to study and analyze the composition and evolution of organisms. Currently commonly used dehydrating agents and clearing agents are compounds such as absolute ethanol and chloroform, which are expensive and partially toxic. In addition, the current preparation of specimens of cells and microorganisms is usually given a given ratio, and only one final state is produced, lacking gradual research and detailed analysis of the entire process, so many phenomena are only half-understood. Contents of the inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28G01N1/2813
Inventor 吴敏胡婧
Owner 吴敏
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