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Method for identifying common vegetable jute varieties based on ISSR fingerprint spectrum system and application thereof

A fingerprint and jute technology, applied in the field of crop genetics and breeding, can solve the problems such as the inability to realize rapid identification and the like

Pending Publication Date: 2021-08-20
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, because the living habits, plant traits, and genetic genes of these five commonly used jute varieties are relatively similar, it is not possible to quickly identify them simply by looking at their appearance or by simply relying on a single gene.

Method used

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  • Method for identifying common vegetable jute varieties based on ISSR fingerprint spectrum system and application thereof
  • Method for identifying common vegetable jute varieties based on ISSR fingerprint spectrum system and application thereof
  • Method for identifying common vegetable jute varieties based on ISSR fingerprint spectrum system and application thereof

Examples

Experimental program
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Embodiment 1

[0027] 1. Extraction of Vegetable Jute Total DNA

[0028] The tips, mortar, and extraction buffer used in the extraction process of vegetable jute total DNA were first autoclaved for 20 minutes, and then taken out for later use. Extract DNA according to the following steps:

[0029] (1) Preheat CTAB (0.1 mol / L Tris-HCl pH8.0, 0.025 mol / L EDTA-Na2pH8.0, 1.4 mol / L NaCl, 2% CTAB (w / v)) extract in a 65°C water bath ;

[0030] (2) Grind 2 to 3 g of fresh or -80°C frozen leaves in liquid nitrogen;

[0031] (3) Quickly add CTAB extract, mix well, pour into a centrifuge tube, and place in a water bath at 65°C for 30 min, while shaking gently;

[0032] (4) Add an equal volume of chloroform / isoamyl alcohol, mix well, and centrifuge at 12000 rpm for 10 min at room temperature;

[0033] (5) Take the supernatant, add 5M NaCl and absolute ethanol according to the ratio of extract: 5M NaCl: dehydrated ethanol = 1:1:1 to prepare a mixture, invert and mix well;

[0034] (5) Aspirate the su...

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Abstract

The invention belongs to the field of crop genetic breeding, and relates to rapid screening of vegetable jute germplasms, in particular to a method for identifying common vegetable jute varieties based on an ISSR fingerprint spectrum system and application thereof. The method comprises the following steps of: extracting total DNA of to-be-detected vegetable jute; carrying out PCR amplification by taking the total DNA of the jute as a template and sequentially taking a No.1 primer, a No.2 primer and a No.3 primer in an ISSR primer identification group as ISSR primers; and analyzing strips after PCR amplification, establishing a DNA fingerprint spectrum according to strip information presented by each primer in the ISSR primer identification group of the common vegetable jute varieties, and identifying the variety of the to-be-detected vegetable jute. In the invention, five vegetable jute varieties can be completely distinguished through the polymorphism of spectral bands amplified by the ISSR primers UBC814, UBC854, UBC872, UBC874 and UBC878, and the specificity and accuracy of the DNA amplification strips are greatly improved by raising the annealing temperature.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to rapid screening of vegetable jute germplasm, in particular to a method for identifying common vegetable jute varieties based on an ISSR fingerprint system and an application thereof. Background technique [0002] Vegetable jute is an annual herbaceous plant of the genus Corchorus in the Tiliaceae family, and belongs to the edible species of jute (Corchorusolitorius L.) crops. Vegetable jute mainly eats tender stems and young leaves. The tender stems are crisp and crisp, and the young leaves are soft and fragrant, with unique flavor and excellent taste. Regular food has the functions of supplementing calcium, selenium, trace elements, strengthening the spleen and stomach, laxative and laxative, and lowering blood pressure. , anti-fatigue, anti-cancer and other effects (Li Chuying et al., 2015). There are very few pests and diseases in the cultivation process of vegetable j...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/156
Inventor 廖小芳赵艳红侯文焕唐兴富
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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