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Bacillus pumilus with a denitrification function and application of bacillus pumilus

A technology of bacillus and sea rod, applied in the field of microbiology and environmental engineering

Active Publication Date: 2021-08-24
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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However, there is no relevant patent on the sea bacillus with denitrification function and its application

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  • Bacillus pumilus with a denitrification function and application of bacillus pumilus

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Embodiment 1

[0023] 1. Isolation, Purification and Preservation of Bacillus marinum S33H4

[0024] Southwest Indian Ocean deep-sea sediment samples (S38°42′54″; E46°58′6″), two sediment samples were taken, connected to a Erlenmeyer shaker flask containing 100mL enrichment medium, 180rpm, 28°C, carried out Enrichment culture. After culturing for 5 days, transfer the culture solution to a new Erlenmeyer flask containing the enrichment medium at a ratio of 1:100 by volume, at 28°C, 180 rpm, and culture for 5 days for enrichment twice, repeat the above steps for the third time Enrichment. The third enriched culture solution was diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 , take 10 -3 、10 -4 、10 -5 100 μL of the diluted culture solution was spread on the separation medium, and cultured in a constant temperature incubator at 28°C for 3-5 days. The colony shape, color, etc. were observed with the naked eye, and a single colony on the plate was picked and purified by streaking. The puri...

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Abstract

The invention discloses bacillus pumilus with a denitrification function and application of the bacillus pumilus. The preservation number of the Marivirgasp. (Marivirgasp.) S33H4 is GDMCC (China General Microbiological Culture Collection Center) No.61709, and the preservation number of the Marivirgasp. The bacillus pumilus S33H4 can effectively remove nitrate nitrogen, ammonium nitrogen and nitrite nitrogen in a water body, the removal effect on the nitrate nitrogen and the removal effect on the ammonium nitrogen are both 90% or above, and in addition, the strain also has a remarkable removal effect on the nitrite nitrogen. Therefore, the bacillus pumilus S33H4 has a very good application potential in the denitrification of the water body.

Description

Technical field: [0001] The invention belongs to the technical field of microbes and environmental engineering, and in particular relates to a sea bacillus with denitrification function and its application. Background technique: [0002] With the rapid development of the domestic aquaculture industry, the high-density farming model is more and more popular. However, while high-density farming meets people's demand for aquatic products, it also causes serious harm to the breeding environment and surrounding ecological environment due to factors such as excessive feed input and frequent use of antibacterial agents. On the one hand, the deterioration of the aquaculture water itself, such as the accumulation of excessive bait, feces and secretions, increases the concentration of ammonium nitrogen and nitrite nitrogen in the water, endangering the healthy growth of aquaculture animals, affecting the production of aquaculture, and improving the quality of aquaculture. cost; on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/163C02F2101/166C02F2101/16
Inventor 姚雨欣
Owner JINAN UNIVERSITY
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