Application of trillium tschonoskii total saponins in preparation of medicine for treating arthritis

A technology of trillium and total saponins, which can be used in drug combinations, antipyretics, anti-inflammatory agents, etc., can solve problems such as unclear etiology

Inactive Publication Date: 2021-08-31
CHINA THREE GORGES UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many inflammatory factors such as TNF-α, IL-6 and IL-1β are involved in the pathogenesis of RA, but the exact etiology is still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of trillium tschonoskii total saponins in preparation of medicine for treating arthritis
  • Application of trillium tschonoskii total saponins in preparation of medicine for treating arthritis
  • Application of trillium tschonoskii total saponins in preparation of medicine for treating arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The extraction method of trillium total saponins is to take trillium pearls and dry them in a constant temperature oven at 48°C, crush them with a traditional Chinese medicine grinder and pass them through a 60-mesh sieve, use methanol as a solvent, use a multifunctional seepage tank, normal pressure, 60°C Seepage extraction, once every 2 hours, repeated several times. The extract was concentrated under reduced pressure and freeze-dried to obtain the total trillium extract. Take an appropriate amount, add water to dissolve, extract with petroleum ether, ethyl acetate and n-butanol in sequence, concentrate under reduced pressure, and freeze-dry to obtain the n-butanol extract, which is the total saponins of Trillium. The TTM solution was prepared with carboxymethylcellulose sodium physiological saline to make the concentration 20%, sonicated for 30 minutes, and stored in a refrigerator at 4°C.

[0022] Tripterygium glycosides were purchased from Zhejiang Deende Pharmace...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an application of trillium tschonoskii total saponins in preparation of a medicine for treating rheumatoid arthritis. The effect of the trillium tschonoskii total saponins on adjuvant-induced arthritis model rat inflammation is researched from an NOD-like receptor protein 3 / cysteine aspartic protease 1 pathway. The SD rats are randomly divided into a blank group, a model group, a tripterygium glycosides group, a trillium tschonoskii total saponin low-dose group, a trillium tschonoskii total saponin medium-dose group and a trillium tschonoskii total saponin high-dose group, and each group comprises six rats. The result shows that compared with the Control group, the foot swelling degree, the arthritis score, the serum TNF-alpha, IL-1beta and IL-6 levels of the AA group, the NLRP3 protein expression level in synovial tissues and the NLRP3, ASC and Caspase-1 protein levels in ankle joints are all remarkably improved; knee joint synovial cell proliferation, interstitial edema and inflammatory cell infiltration of the AA group rats; and after treatment with TG and TTM with different doses, the indexes are all improved to different degrees.

Description

technical field [0001] The invention relates to the application of trillium total saponins as a medicine for treating rheumatoid arthritis. It belongs to the field of biopharmaceuticals. Background technique [0002] Rheumatoid arthritis (RA) is an autoimmune inflammatory disease characterized by synovitis, clinically manifested as pain, swelling, multiple joint stiffness, fever, fatigue, and involving extra-articular organs, such as Interstitial pneumonia. After the onset, the joint destruction progresses rapidly, and once the affected joint deforms, irreversible physiological dysfunction occurs. Many inflammatory factors such as TNF-α, IL-6 and IL-1β are involved in the pathogenesis of RA, but the exact etiology is still unclear. Recent studies have shown that the mRNA expression of NOD-like receptor 3 (NLRP3) and related inflammatory factors are up-regulated in monocytes, macrophages and dendritic cells in the peripheral blood of RA patients, so the progression of rheu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61P19/02A61P29/00
CPCA61K36/896A61P19/02A61P29/00A61K2236/333A61K2236/35A61K2236/51
Inventor 喻玲玲谭晓茜柳蔚李世刚史伟文蒋安蓉
Owner CHINA THREE GORGES UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap