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Application of RPS9 protein in predicting good response of cynomolgus monkey to superovulation

A technology of superovulation and cynomolgus monkeys, which is applied in the field of superovulation and can solve the problems that the interaction is not yet clear

Active Publication Date: 2021-09-03
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on the relationship between RPS9 protein and superovulation has not been reported,

Method used

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  • Application of RPS9 protein in predicting good response of cynomolgus monkey to superovulation
  • Application of RPS9 protein in predicting good response of cynomolgus monkey to superovulation

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Embodiment Construction

[0023] Specific embodiments of the present invention will be further described below. It should be noted here that the descriptions of these embodiments are used to help understand the present invention, but are not intended to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below may be combined with each other as long as they do not constitute a conflict with each other.

[0024] The test methods used in the following experimental examples are conventional methods unless otherwise specified; the materials and reagents used are commercially available reagents and materials unless otherwise specified.

[0025] 1. Superovulation experiment process

[0026] Observe the physiological state of adult female cynomolgus monkeys in the target monkey group every day, and register the cage number and monkey number on the first day when menstruation occurs. On the 1st to 4th days of menstruation, ve...

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Abstract

The invention relates to application of RPS9 protein in predicting good response of cynomolgus monkeys to superovulation, and belongs to the technical field of superovulation. Biological information analysis is carried out on blood sample protein on the first day of a superovulation success group, the fifth day of the superovulation success group, the first day of a superovulation failure group and the fifth day of the superovulation failure group through adoption of a proteomics method, and differential proteins among the groups are obtained. The protein expression change of the blood of the superovulation monkeys is discussed from protein level research. In superovulation of cynomolgus monkeys, RPS9 protein is expressed on the fifth day in a superovulation success group, and is not expressed in a superovulation failure group on the fifth day. It is prompted that down-regulation of PRS9 expression in a superovulation failure group affects activation of an MAPK signal channel and an NK-kappa B channel, so that cell apoptosis, cell cycle arrest and proliferation inhibition are achieved. The down-regulation of PRS9 expression may cause activation of p53, so that cell proliferation is inhibited, follicle formation is affected, and superovulation response fails. Therefore, the RPS9 protein becomes a new molecule for predicting good response of the cynomolgus monkey to superovulation, and has practical application value.

Description

technical field [0001] The invention relates to the technical field of superovulation, and more specifically, relates to the application of RPS9 protein in predicting good response to superovulation in cynomolgus monkeys. Background technique [0002] Superovulation refers to the use of exogenous hormones to stimulate the ovaries of female animals, to promote the simultaneous growth and development of multiple follicles in the ovary, and finally to obtain multiple mature oocytes. In cynomolgus monkeys, only one dominant follicle matures and ovulates during the natural menstrual cycle. If the superovulation technique is used during the menstrual period, the number of oocytes per ovulation can be increased from 1 to 10-20 or even more. Mature oocytes are important materials for research on human diseases, genetic engineering and embryo engineering. However, the number of qualified oocytes obtained through natural cycle is extremely small, which is far from meeting the experi...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/47G01N2800/36
Inventor 杨世华侯润杰赵鑫源
Owner SOUTH CHINA AGRI UNIV
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