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Process for preparing pectinase for degumming ramie and its application in degumming ramie

A technology of degumming enzyme and ramie, which is applied in hydrolytic enzymes, chemical post-treatment of fibers, textiles and papermaking, etc. It can solve the problems of low pectinase activity, long degumming time, difficult application, etc.

Inactive Publication Date: 2004-01-21
SHANDONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Chinese patent 97109044 discloses a strain of alkalophilic bacillus and its ramie degumming method, but because the pectinase activity produced by it is relatively low, the degumming time is long (16-24 hours), and it is also difficult to apply in industry

Method used

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Examples

Experimental program
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Embodiment

[0018] The process flow implemented by the present invention includes:

[0019] Strain breeding→strain cultivation→enzyme production by strains→enzyme production by tertiary fermentation→crude enzyme solution;

[0020] Ramie tying the cage → soaking enzyme treatment → washing hemp → copying hemp → rinsing → oiling → degumming → drying.

[0021] Naturally collected bacterial samples from the soil in Jinan, and subjected to initial shake flask screening and re-screening from nearly dry strains. The enzyme activity was determined according to Nelson Somogyi's method, and a laboratory numbered No.13 strain was selected, namely Bacillus subtilis Bacillus subtilis, deposit number CCTCCNO: M200038, its biological characteristics are: the bacteria are rod-shaped, uniformly stained, G+, endospores grow proximally, and form an ellipse, the sporangia is smaller than the bacteria, aerobic, over Catalase is positive and grows well in broth containing 7% NaCl.

[0022] After the Bacillus subtil...

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PUM

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Abstract

A pectinase for degumming remie is prepared through naturally sampling bacteria, measuring its enzyme activity by Nelson Somogyi method, and sceening the bacteria. It is Bacillus subtilis (CCTCC0 No.M200038). Its advantages are simple culture condition, high growth and reproduction speed, and high activity to generate pectinase. Its fermenting liquid can be directly used to degum the ramie with short period (2-4 hr), high degamming rate (97.5-98.5% and no damage to fibres.

Description

Technical field: [0001] The invention relates to the production and application of an enzyme, in particular to a degumming pectinase, its industrialized production method and its application in a ramie degumming process. Background technique: [0002] Ramie (Bochmeria nivea) belongs to the Urticaceae (Urticaceae) perennial, perennial herb, native to my country, and is called "Chinese grass" by European and American countries. The output accounts for about 80% of the world. Ramie fiber is the thinnest and longest among the hemp fibers, with a content of about 70%. It is an important raw material for the textile industry. Because about 25-35% of the glue (mainly pectin, hemicellulose, lignin, etc.) is attached to the fiber. Therefore, to obtain industrially usable fibers, degumming treatment must be carried out, and the degumming treatment of fibers is directly related to the development and utilization of fibers. For decades, people have tried to change the environmental pollution...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12S3/04D01C1/00
Inventor 刘自镕王冬毛任建平杨光辉冯瑞良李雪梅
Owner SHANDONG UNIV
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