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UPLC-MS/MS detection method for multiple medicines added in traditional Chinese veterinary medicine powder

A detection method and technology for veterinary drugs, which can be applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of illegal addition, excessive residues of livestock and poultry meat, eggs and milk, and bacterial resistance, and achieve the effect of low detection limit.

Pending Publication Date: 2021-11-02
魏秀丽 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Amoxicillin, penicillin-V, ampicillin, ceftiofur hydrochloride, oxacillin, and cloxacillin are all β-lactam drugs with strong broad-spectrum antibacterial effects. Metronidazole is an anti-anaerobic drug, rimantadine is an antiviral drug, warnemulin and metronidazole have strong antibacterial effects, and rimantadine has antiviral effects to a certain extent , can enhance the therapeutic effect of traditional Chinese medicine powder, will be used to illegally add to traditional Chinese medicine, used without knowing it, can not use the drug safely according to the drug withdrawal period, resulting in excessive residues of livestock and poultry meat, egg and milk, causing bacterial resistance Drug production, multiple allergic reactions, and other adverse reactions

Method used

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  • UPLC-MS/MS detection method for multiple medicines added in traditional Chinese veterinary medicine powder
  • UPLC-MS/MS detection method for multiple medicines added in traditional Chinese veterinary medicine powder
  • UPLC-MS/MS detection method for multiple medicines added in traditional Chinese veterinary medicine powder

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Effect test

example 1

[0071] Example 1: UPLC-MS / MS detection method for adding a variety of drugs in medium beast drugs, the specific steps are as follows:

[0072] (1) Sample treatment method, taking a negative medium and beast dispersion 250 mg, to 15 ml of centrifuge tube, 10 ml of 10% aqueous aqueous solution, shake the bed shock, centrifugal 10000 rpm / min, 5 min, and taking the supernatant by filtering with 0.20 μm filter, 0.1 ml of the filtrate, added to 10 ml by the initial flow, then take 0.1 ml, add the base to 10 ml, and the matrix matches the blank solution; the positive sample is synthesized, and the test;

[0073] (2) Take Amoxi, penicillin-V, ampicillin, hydrochloride cefoli, phenylazole, thromazole, Wali Miaolin, metronidazole, diamineacetine 10 phlegm 10 mg, 10 The aqueous acetonitrile solution is configured to be found in 0.1 mg / ml of reserve, and is diluted with a matrix blank solution to 100 ng / ml, 75 ng / ml, 50 ng / ml, 20 ng / ml, 10 ng / ml, 5 ng / ml, 2 ng / ML test;

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Abstract

The invention relates to a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) detection method for multiple medicines added in traditional Chinese veterinary medicine powder, and belongs to the technical field of detection methods for medicines added in traditional Chinese medicines. According to the method, the defects of microscopic identification and thin-layer identification are broken through, ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) is adopted, parent ions and daughter ions are optimized, and mass spectrometry parameters are adjusted, 10 illegally added drugs such as amoxicillin, penicillin-V, ampicillin, ceftiofur hydrochloride, oxacillin, cloxacillin, valnemulin, metronidazole and rimantadine can be qualitatively and quantitatively detected. According to the method, parent ions and specific daughter ion fragments are used as identity characteristics of different compounds, uniqueness and specificity are achieved, the sensitivity is one thousandth to one ten thousandth of that of a liquid chromatography method, and the detection limit is low.

Description

Technical field [0001] The present invention relates to a veterinary drug powders added more drugs UPLC-MS / MS detection method, a method belonging to the technical field of detection of traditional Chinese medicine drug addition. Background technique [0002] The present invention is applicable to veterinary drug powders (Powder dredging leaves Alice, dysentery Chuankangping scattered, polyester antitussive drug powder, powder, etc. Maxinshigan) was added amoxicillin, -V penicillin, ampicillin, ceftiofur hydrochloride, oxacillin, cloxacillin, valnemulin, metronidazole, measured rimantadine. [0003] Alice leaves dredging scattered with detoxification, blood circulation, Sanjie swelling effect, often used in the treatment of acute mastitis in dairy cows, included in the 2017 edition veterinary medicine quality standard volume. Prescription ingredients are 816 grams forsythia, Folium 396 g, 396 g safflower, Millettia 816 grams, 816 grams of honeysuckle, Fritillaria 388 grams. 6 o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/72
CPCG01N30/02G01N30/06G01N30/54G01N30/72
Inventor 魏秀丽张传津冯言言战余铭张洪芳汪安国王尚明
Owner 魏秀丽
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