Akecmann acetyl hexosidase Amuc_2109 protein prepared by genetic engineering and preparation method and application thereof

A bacterial acetylhexosidase and genetic engineering technology, applied in the field of protein engineering, can solve problems such as scarcity of structural data

Pending Publication Date: 2021-11-12
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, structural data on representative enzymes of A. muciniphila for degrading mucins are still scarce

Method used

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  • Akecmann acetyl hexosidase Amuc_2109 protein prepared by genetic engineering and preparation method and application thereof
  • Akecmann acetyl hexosidase Amuc_2109 protein prepared by genetic engineering and preparation method and application thereof
  • Akecmann acetyl hexosidase Amuc_2109 protein prepared by genetic engineering and preparation method and application thereof

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Experimental program
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Embodiment Construction

[0027] The application will be described in further detail below in conjunction with the accompanying drawings. It is necessary to point out that the following specific embodiments are only used to further illustrate the application, and cannot be interpreted as limiting the protection scope of the application. The above application content makes some non-essential improvements and adjustments to this application.

[0028] 1. Materials

[0029] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0030] 2. Method

[0031] 2.1 Construction, expression and purification of Ekkermansia Amuc_2109 protein

[0032] 2.1.1 Construction of recombinant plasmids

[0033] (1) Extract the total RNA of A.muciniphila and reverse transcribe it into cDNA, use the cDNA as a template, amplify the DNA fragment of the A...

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Abstract

The invention relates to the technical field of protein engineering, and particularly discloses an Akkermann acetyl hexosidase Amuc_2109 protein prepared by genetic engineering and a preparation method and application thereof. The Amuc_2109 protein has any one of the following sequences: (1) an amino acid sequence as shown in SEQ ID NO.1; and (2) a homologous protein sequence which has an amino acid sequence obtained by substituting, deleting and/or adding one or more amino acid sequences to the amino acid sequence of (1) and has the same function as the amino acid sequence of (1). The medicinal value of the Amuc_2109 protein is proved by purifying the wild type and mutant protein of the Amuc_2109 and playing a role in improving the DSS-induced colitis through the purification of the wild type and mutant protein of the Amuc_2109.

Description

technical field [0001] The invention belongs to the technical field of protein engineering, and in particular relates to an Ekkermansia acetylhexosidase Amuc_2109 protein prepared by genetic engineering, a preparation method and application thereof. Background technique [0002] Akkermansia muciniphila (A.muciniphila) is a Gram-negative anaerobic bacterium belonging to the verrucomicrobial phylum that resides stably in the outer layer of the human gastrointestinal tract (GI). A.muciniphila can improve colitis, and in addition A.muciniphila SCFAs produced by fermentation are inversely correlated with inflammation. Outer membrane protein Amuc_1100 can maintain structure at high temperature, interact with TLR2, and improve intestinal barrier function. A. muciniphila can increase the number of goblet cells and the production of antimicrobial peptides to enhance intestinal barrier function. Therefore, in recent years, A.muciniphila has received extensive attention in human inte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24C12N15/70C12N1/21A61K38/47A61P1/04C12R1/19
CPCC12N9/2402C12N15/70A61P1/04C12Y302/01052A61K38/00
Inventor 张敏钱锴钺王永中盛康亮王明珠
Owner ANHUI UNIVERSITY
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