Application of schisandra polysaccharide in preparation of medicine or health product for treating inflammatory bowel disease

A technology of inflammatory bowel disease and schisandra, applied in the field of biopharmaceuticals and molecular biology, can solve the problems of multiple side effects and insignificant effects, and achieve the effect of improving colitis and inflammatory bowel disease

Active Publication Date: 2021-08-17
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that existing drugs for improving inflammatory bowel disease have many side effects and the curative effect is not significant, and provide a polysaccharide extract from Schisandra chinensis and its application in improving inflammatory bowel disease

Method used

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  • Application of schisandra polysaccharide in preparation of medicine or health product for treating inflammatory bowel disease
  • Application of schisandra polysaccharide in preparation of medicine or health product for treating inflammatory bowel disease
  • Application of schisandra polysaccharide in preparation of medicine or health product for treating inflammatory bowel disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 A preparation method of an extract capable of improving inflammatory bowel disease

[0036] Weigh 400 g of each dried sample of Schisandra chinensis in raw vinegar, reflux three times the amount (1200 mL) of petroleum ether for 4 hours to degrease, and remove the petroleum ether after degreasing. Then add pure water (3200mL) in an amount 8 times the weight of the decoction pieces, heat to reflux at 100°C, extract twice for 3 hours each time, and combine the extracts. Concentrate the water extract to 200mL, add absolute ethanol to make the alcohol content reach 80%, let stand overnight, and centrifuge to obtain the supernatant filtrate and precipitate, which is Schisandra crude polysaccharide.

[0037] Deproteinization by Savage method: dissolve the crude polysaccharide of Schisandra chinensis with pure water to 400mL, because the protein in chloroform can be deformed and precipitated, add chloroform and n-butanol in the ratio of polysaccharide solution:chlorof...

Embodiment 2 5

[0039] Determination of total polysaccharide content in embodiment 2 Schisandra polysaccharide extract

[0040] Take 1 mg of schisandra polysaccharide freeze-dried powder refined in Example 1, add distilled water to make the volume to 10 mL, that is, the concentration of the sample solution is 0.1 mg / mL. Using glucose as a control, the total polysaccharide content was determined at 490nm by phenol-sulfuric acid method and ultraviolet spectrophotometer. According to calculation, the total polysaccharide content in the refined Schisandra polysaccharide extract is 85-95%, as shown in Table 1.

[0041] The polysaccharide content (X±SD, n=3) in each batch of Schisandra chinensis polysaccharide extract sample of table 1

[0042]

[0043]

Embodiment 3 5

[0044] Example 3 Study on the Effect of Schisandra Polysaccharide Extract on Colitis Mice

[0045] The Schisandra polysaccharide extract was obtained by the extraction method in Example 1, and two extract solutions of different concentrations (ie high and low doses) were prepared, the low dose: 0.2 g / mL, and the high dose: 0.4 g / mL.

[0046] C57BL6 mice, body weight 20.0+2.0g. All animals were fed under (SPF) grade and adaptively fed for one week. DSS was made into 2% DSS and 3.5% DSS aqueous solution with sterilized distilled water, instead of drinking water, and the experimental C57BL6 mice were allowed to drink freely to induce colitis. 2% DSS aqueous solution was given on the 1st-3rd day, and 3.5% DSS aqueous solution was replaced on the 4th-7th day.

[0047] The experimental mice were fed adaptively for one week, and were randomly divided into 8 groups. The normal group (Normal): drank drinking water without DSS for 21 days. Model group (DSS): Mice acquired acute colit...

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Abstract

The present invention relates to the new application of Schisandra chinensis total polysaccharides in the preparation of medicines or health products for treating inflammatory bowel disease, specifically for the preparation of medicines for treating ulcerative colitis (UC), Crohn's disease (CD) and acute and chronic intestinal diseases. New uses in medicine or health products. Experiments have shown that the total polysaccharides of Schisandra chinensis can significantly improve the inflammatory damage of the colon, reduce the spleen index, increase the thymus index, and effectively reduce the content (activity) of MPO, NO, MDA, and ROS in the colon, while increasing the content of GSH and SOD. At the same time, Schisandra polysaccharides regulated the composition and diversity of intestinal microorganisms in mice with colitis, significantly increased the relative abundance of Firmicutes, Saccharomycetes, Enterorhabdus, etc., and decreased the relative abundance of Firmicutes, Turicibacter, Akkermansia, etc. degree, and significantly increased the content of propionic acid, butyric acid and isobutyric acid in the colon, suggesting that the total polysaccharides of Schisandra chinensis can be used to prepare drugs for treating or improving inflammatory bowel disease.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals and molecular biology, and in particular relates to an extract of Schisandra chinensis total polysaccharide and its application in improving inflammatory bowel disease. Background technique [0002] Inflammatory bowel disease (IBD) mainly includes inflammatory bowel disease (UC) and Crohn's disease (CD), which are chronic diseases characterized by intestinal inflammation and epithelial damage. Recurrent disease is difficult to cure and poses a serious threat to human health. In recent years, the incidence of IBD has been increasing year by year, gradually evolving into a global disease. In addition, epidemiological data show that the duration and severity of chronic colitis are important risk factors for the induction of colitis-associated colorectal cancer. The main pathological mechanism of inflammatory bowel disease is still not very clear, and its pathogenesis is multifaceted, suc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/79A61K36/57A61P1/00A61P29/00A23L33/125A23L33/105C08B37/00
CPCA23V2002/00A61K31/715A61K36/57A61K36/79A23L33/105A23L33/125A61P1/00A61P29/00C08B37/003A23V2200/32A23V2250/21A23V2250/51A23V2300/14A23V2300/40A23V2300/20A23V2300/38
Inventor 陆兔林苏联麟毛春芹季德顾薇李昱王巧晗毛靖
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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