Application of tumor mutation load detection reagent based on circulating tumor DNA in preparation of kit for predicting T cell lymphoma prognosis

A mutation load and detection reagent technology, applied in the field of biomedicine, can solve the problems that are difficult to promote, and achieve the effect of simple and easy method, stable method and broad application prospect

Pending Publication Date: 2021-12-03
JINAN UNIVERSITY
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Problems solved by technology

However, due to the cost of whole exome sequencing (WES) and its timeliness and informatics challenges in the clinical setting; thus, it is difficult to scale up clinically

Method used

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  • Application of tumor mutation load detection reagent based on circulating tumor DNA in preparation of kit for predicting T cell lymphoma prognosis
  • Application of tumor mutation load detection reagent based on circulating tumor DNA in preparation of kit for predicting T cell lymphoma prognosis
  • Application of tumor mutation load detection reagent based on circulating tumor DNA in preparation of kit for predicting T cell lymphoma prognosis

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Experimental program
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Embodiment 1

[0051] (1) Peripheral blood was collected under the premise of signing an informed consent form with the patient. Peripheral blood samples from 79 adult patients with first-onset TCL in the Lymphoma Department of Guangdong Provincial People's Hospital were collected. All samples were taken from peripheral blood heparin anticoagulation at the time of admission. This part of the research protocol has been approved by the ethics committee of the unit. At the same time, clinical data such as survival time and survival status of TCL patients were collected.

[0052] (2) Extraction of plasma circulating tumor DNA

[0053] 2.1 Add the blood sample to a 50mL centrifuge tube, centrifuge at 3000g for 5-10 minutes, absorb the upper 10mL plasma into a new 50mL centrifuge tube;

[0054] 2.2 Add 1mL QIAGEN Proteinase K to the tube;

[0055] 2.3 Add 8mL buffer ACL and mix well for 30 seconds;

[0056] 2.4 Incubate at 60°C for 30 minutes;

[0057] 2.5 Add 18mL buffer ACB to the lysate in ...

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Abstract

The invention provides application of a tumor mutation load detection reagent based on circulating tumor DNA in preparation of a kit for predicting T cell lymphoma prognosis. The inventor of the invention finds that tumor mutation load (panel-TMB13) calculated by mutation of exons of 13 genes in plasma circulating tumor DNA of a patient with T-cell lymphoma is related to prognosis of the patient with T-cell lymphoma for the first time. When it is found that the panel-TMB13 is more than 2 exon non-synonymous mutation, the possibility of poor prognosis of TCL patients is high. The invention also finds that the risk stratification constructed by combining panel-TMB13 with the international prognostic index is better than the existing risk stratification based on the international prognostic index, more accurate risk stratification can be carried out on the TCL patient, and the invention has important guiding significance for prognosis judgment of the TCL patient and formulation of a clinical treatment scheme.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of a tumor mutation load detection reagent based on circulating tumor DNA in the preparation of a kit for predicting the prognosis of T-cell lymphoma. Background technique [0002] T-cell lymphoma (TCL) originates from lymphoblasts or mature T cells, and is a highly aggressive and heterogeneous disease. TCL accounts for 10-15% of non-Hodgkin's lymphomas and can be further subdivided into many subtypes. Current treatment options, such as chemotherapy, radiotherapy, targeted therapy, etc., can improve the complete remission rate of TCL patients; however, most of them still have a poor prognosis due to recurrence and progression, partly due to heterogeneity with TCL. qualitatively related. This heterogeneity manifests itself at the clinical level as well as genetics. However, the current international prognosis index (IPI) risk stratification based on clinic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12Q1/6886G16B15/30G16B20/50
CPCC12Q1/6869C12Q1/6886G16B15/30G16B20/50C12Q2600/118C12Q2600/156C12Q2535/122
Inventor 李扬秋陈存特黄玲陈政欧秋翔刘思初江新苗陈菲莉魏小娟郭汉国邵阳曾成武李文瑜
Owner JINAN UNIVERSITY
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