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Application of autoantibody in preparation of pancreatic ductal adenocarcinoma diagnostic kit

A technology of pancreatic ductal adenocarcinoma and a diagnostic kit, which is applied in the field of medical biological detection, can solve the problems of PDAC diagnosis sensitivity, specificity, non-systematic research, high-throughput screening research of PDAC autoantibodies, etc., and achieve clinical reference value and high reliability, simple detection method, and high patient compliance

Pending Publication Date: 2021-12-10
FUDAN UNIV SHANGHAI CANCER CENT
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Studies have found that multiple autoantibodies (such as anti-Ezrin, p53, p16, p62, survivin, Koc, IMP1, etc.) are related to the development of PDAC [9-10] , but the sensitivity and specificity of these autoantibodies and their combinations for the diagnosis of PDAC have not been systematically studied, and there is still no high-throughput screening research on PDAC-related autoantibodies

Method used

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  • Application of autoantibody in preparation of pancreatic ductal adenocarcinoma diagnostic kit
  • Application of autoantibody in preparation of pancreatic ductal adenocarcinoma diagnostic kit
  • Application of autoantibody in preparation of pancreatic ductal adenocarcinoma diagnostic kit

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Embodiment 10

[0029] Example 10 Autoantibody Screening and Verification

[0030] The screening and verification process in this embodiment is as follows: figure 2 As shown, the details are as follows:

[0031] 1. Initial screening stage

[0032] Serum samples from 30 PDAC patients and 30 healthy people were collected, and each sample was detected using a HuProtTM protein chip. The detection principle is as follows: figure 1 Shown: Chip-specific antibodies (including IgG, IgM or other types of antibodies) bind to proteins immobilized on the chip, wash to remove unbound antibodies and other proteins, and then use anti-human IgM fluorescently labeled secondary antibody (cy5 labeled , appearing in red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, appearing in green), the signal was read by a fluorescent scanner, and the strength of the signal was positively correlated with the affinity and quantity of the antibody. Data were extracted and normalized for analysis.

[0033...

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Abstract

The invention relates to the technical field of medical biological detection, provides novel application of ten autoantibodies, namely anti-TSPAN18, ENTPD1, IZUMO4, DHRS7C, ATP5G1, SECTM1, CPT1A, SLC39A14, SLC7A4 and LDLRAD4 antibodies, in particular to application in preparation of a diagnostic reagent or a diagnostic kit for pancreatic ductal adenocarcinoma, and further provides a kit for diagnosing pancreatic ductal adenocarcinoma by detecting the ten autoantibodies. The kit and the detection method disclosed by the invention are simple, convenient, reliable, short in period, high in specificity and easy to clinically popularize.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, and relates to the application of 10 kinds of autoantibodies in the diagnosis of pancreatic ductal adenocarcinoma. Background technique [0002] Pancreatic ductal adenocarcinoma (Pancreatic ductal adenocarcinoma, PDAC) is one of the most common malignant tumors in my country. About 90% of pancreatic cancers are ductal adenocarcinoma originating from the ductal epithelium. It has high incidence, difficult early diagnosis, early metastasis and recurrence. , poor prognosis and other characteristics, the 5-year survival rate is only 9% [1,2] . Surgical resection is the first choice and the only radical treatment for early PDAC, but about 85% of PDAC are locally advanced or accompanied by distant metastasis when diagnosed, and cannot be surgically removed. Chemotherapy is the main treatment for these patients, but it is easy to tolerate medicine, poor efficacy [3] . Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57488G01N33/57438
Inventor 庄丽萍孟志强王鹏
Owner FUDAN UNIV SHANGHAI CANCER CENT
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