High-frequency Differentiation Medium of Caoyulu Callus and the Culture Method of Caoyulu Callus

Abstract

The invention discloses a high-frequency differentiation medium of Caoyulu callus, which is composed of inorganic salt, organic components, carbon source, additives, coagulant and hormone, wherein the inorganic salt contains NH 4 NO 3 , KNO 3 , CaCl 2 , KH 2 PO 4 , MgSO 4 , KI, etc., the organic components contain Na 2 EDTA, inositol, niacin, thiamine hydrochloride, pyridoxine hydrochloride and glycine, the carbon source is sucrose, the additive is Caoyulu callus extract, the coagulant is crystal agar, and the hormones are 6‑BA and NAA. The medium can significantly improve the differentiation ability of cells, realize the high-frequency differentiation and regeneration of callus, and form a well-grown grass-gyulu plant under the condition of no root system. There is no need to change the medium type during the entire culture period, which significantly shortens the The time for callus redifferentiation is shortened, and a large number of easy-to-survive tissue culture seedlings are obtained, and the cultivation process is not limited by seasons, which effectively improves the reproduction efficiency and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-frequency differentiation medium for the callus of Liliaceae succulent succulent succulent plant and a method for cultivating the callus of the plant. Background technique [0002] Haworthiacymbiformis var. obtusa (Haw.) Baker belongs to the Liliaceae genus. It is emerald green all the year round and grows fast. The mature plants are still small in size and beautiful in clusters. It is suitable for indoor planting and is widely loved by succulent lovers. [0003] The young plants of Caoyulu can survive and grow in both rooted and rootless states, and the main propagation methods are ramets and leaf cuttings. These traditional methods are greatly affected by the season and environment, the success rate of reproduction is not high, and the growth of the mother plant is affected to a certain extent. The seedlings of ramets or leaf cuttings grow slowly...

AI Technical Summary

Problems solved by technology

These traditional methods are greatly affected by the season and the environment, the reproductive success rate is not high and have a certain impact on the growth of the mother plant, the seedlings of the ramets or leaf cuttings grow slowly, the reproduction time cycle is long, and the labor and time costs of the whole process are relatively large.

However, the current existing tissue culture methods are directly applied to the propagation of Gylodendron spp., which has low callus differentiation and regeneration efficiency, slow growth of regenerated plants, and requires a long period of rooting culture to form seedlings of Gylodylum syringae suitable for transplantation. Disadvantages of long cultivation period, weak growth of seedlings and high cost

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