High-frequency differential culture medium of Haworthia cymbiformis var obtusa callus and culture method of Haworthia cymbiformis var obtusa callus

Abstract

The invention discloses a high-frequency differential culture medium of Haworthia cymbiformis var obtusa callus. The high-frequency differential culture medium is composed of inorganic salts, organic components, a carbon source, an additive, a coagulator and hormones, wherein the inorganic salts contain NH4NO3, KNO3, CaCl2, KH2PO4, MgSO4, KI and the like, the organic components contain Na2EDTA, inositol, nicotinic acid, thiamine hydrochloride, pyridoxine hydrochloride and glycine, the carbon source is cane sugar, the additive is a Haworthia cymbiformis var obtusa callus extract, the coagulator is gelrite, and the hormones are 6-BA and NAA. According to the culture medium, the differentiation capacity of cells can be remarkably improved, high-frequency differentiation and regeneration of the callus can be achieved, Haworthia cymbiformis var obtusa plants in a good growth state can be formed under the root-system-free condition, the culture medium type does not need to be replaced in the whole culture period, the callus redifferentiation time is remarkably shortened, a large number of tissue culture seedlings easy to survive are obtained, moreover, the cultivation process is not limited by seasons, and the breeding efficiency and economic benefits are effectively improved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-frequency differentiation medium for the callus of Liliaceae succulent succulent succulent plant and a method for cultivating the callus of the plant. Background technique [0002] Haworthiacymbiformis var. obtusa (Haw.) Baker belongs to the Liliaceae genus. It is emerald green all the year round and grows fast. The mature plants are still small in size and beautiful in clusters. It is suitable for indoor planting and is widely loved by succulent lovers. [0003] The young plants of Caoyulu can survive and grow in both rooted and rootless states, and the main propagation methods are ramets and leaf cuttings. These traditional methods are greatly affected by the season and environment, the success rate of reproduction is not high, and the growth of the mother plant is affected to a certain extent. The seedlings of ramets or leaf cuttings grow slowly...

AI Technical Summary

Problems solved by technology

These traditional methods are greatly affected by the season and the environment, the reproductive success rate is not high and have a certain impact on the growth of the mother plant, the seedlings of the ramets or leaf cuttings grow slowly, the reproduction time cycle is long, and the labor and time costs of the whole process are relatively large.

However, the current existing tissue culture methods are directly applied to the propagation of Gylodendron spp., which has low callus differentiation and regeneration efficiency, slow growth of regenerated plants, and requires a long period of rooting culture to form seedlings of Gylodylum syringae suitable for transplantation. Disadvantages of long cultivation period, weak growth of seedlings and high cost

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