Composite amplification system for detecting 129 molecular genetic markers of four types based on NGS technology

Abstract

The invention belongs to the field of innovative research and application of a new forensic medicine technology, and particularly relates to a composite amplification system for detecting multiple molecular genetic markers based on a next-generation sequencing platform. The method comprises the following steps: screening 48 AISNPs for different intercontinental and domestic group forensic medicine progenitor inference and analysis on the basis of an HGDP-CPEH database; 18 multi-allele InDel sites with high polymorphism distribution in domestic populations are screened on the basis of a thousand-person genome plan; 27 micro-haplotypes with high application value in domestic populations are screened based on previous research reports; 34 Y-SNP sites and 2 Y-InDel sites are selected for male individual haplogroup distribution research; and based on a next-generation sequencing platform, synchronous amplification detection analysis of the multiple genetic markers is realized by adopting a multiple PCR method. The system can be used for simultaneously realizing forensic individual identification, genetic relationship identification, mixed sample resolution, forensic initial ancestor analysis and male individual haplogroup distribution research aiming at a field physical evidence inspection material, and can provide more comprehensive and valuable biological information for forensic application research.

Description

technical field [0001] The present invention belongs to the field of research and application of new technologies in forensic medicine, and specifically relates to the disclosure of a compound amplification system of four types of new 129 molecular genetic markers based on a next generation sequencing detection platform (Next generation sequencing, NGS), including system selection ancestry informative single nucleotide polymorphism (AISNP), multi-allelic deletion / insertion polymorphism (insertion / deletion polymorphism, InDel), micro-haplotype and Y chromosome SNP / InDel molecular genetic markers The complex amplification system and its analysis and detection method. Background technique [0002] The analysis of ancestry information in forensic science is beneficial to reveal the biogeographical origin of unknown individuals and to assist judicial investigations by narrowing the scope of investigation. At present, most of the ancestry information analysis systems commonly use...

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Problems solved by technology

However, currently commonly used SNPs and InDels are mostly biallelic variants, and their polymorphisms are low, which is not conducive to the clarification of mixed samples.

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