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Compound as well as mass spectrum standard substance and calibration substance thereof

A compound and standard product technology, which is applied in the field of mass spectrometry, can solve problems such as low accuracy, low signal intensity of fragment ions, and no internal standard standard, and achieve high signal intensity, uniform mass distribution, and abundant fragment ions Effect

Pending Publication Date: 2022-01-14
SHENZHEN BAY LAB
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The calibration of MALDI-ISD depends on the protein standard. The fragment ions produced by the protein standard are diverse and complex, the signal intensity of the fragment ions is not high, and the calibration accuracy is not high. At the same time, this calibration method can only be used for external standard method, mass determination The accuracy is not high
The internal standard method can improve the accuracy of mass determination, but there is currently no internal standard standard available for MALDI-ISD determination

Method used

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  • Compound as well as mass spectrum standard substance and calibration substance thereof
  • Compound as well as mass spectrum standard substance and calibration substance thereof
  • Compound as well as mass spectrum standard substance and calibration substance thereof

Examples

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Effect test

Embodiment 1

[0107] According to the designed polypeptide sequence R o (GDP n ) m , in order to cover the calibration of the mass range of 500-5000Da, Suzhou Qiangyao Biotechnology Co., Ltd. was entrusted to synthesize the peptide RR (GDP 4 ) 8 (SEQ ID No.1), its molecular weight is 4813Da, at the same time in order to illustrate the feasibility of this polypeptide design method, synthesized another polypeptide RR (GDP 3 ) 7 (SEQ ID No.2), its molecular weight is 3573Da. The synthesized polypeptide was taken and prepared into an aqueous solution with deionized water, and the concentration of the polypeptide in the aqueous solution was 0.2 μg / μL. The MALDI matrix used in this embodiment has 1,5-diaminonaphthalene (1,5-DAN, product number 56451, the English name of the manufacturer is Sigma-Aldrich, the Chinese name is Sigma-Aldrich), 2,5- Dihydroxybenzoic acid (super-DHB, product number 50862, Sigma-Aldrich) and α-cyano-4-hydroxycinnamic acid (CCA, product number 70990, Sigma-Aldrich)...

Embodiment 2

[0120] In this example, myoglobin is derived from horse heart, the product number is M1882, and it is purchased from sigma. Using deionized water as solvent, prepare an aqueous solution with a concentration of 0.2 μg / μL.

[0121] Peptide RR (GDP 4 ) 8 Mix with myoglobin at a volume ratio of 1:1 to obtain 1 μL sample, then add 1 μL matrix 1,5-DAN, mix and spot on the MALDI target plate, then perform MALDI-ISD analysis, and simultaneously obtain muscle in the mass spectrum Hemoglobin and RR (GDP 4 ) 8 fragment ions, such as Figure 7 (a), Figure 8 (a) shown. The theoretical mass of the fragment ions of myoglobin was compared with the mass of the fragment ions in the spectrogram, and it was found that the peptide RR (GDP 4 ) 8 Fragment ion-corrected spectra have much larger errors than those corrected by internal standards, such as Figure 7 (b), Figure 8 As shown in (b), in most ions with m / z>1600, the error of the former is greater than 0.2Da, and the error of the l...

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Abstract

The invention discloses a compound as well as a mass spectrum standard substance and a calibration substance thereof. The compound contains at least one chemical bond which is easy to generate fragment ions in mass spectrum analysis. The compound designed by the invention can be used for internal standard correction in mass spectrometry, can also be used for cascade mass spectrometry correction in a positive ion mode and / or a negative ion mode, and is rich in fragment ions, high in signal intensity and uniform in mass distribution.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry analysis, in particular to a class of compounds and mass spectrometry standard products and calibration products thereof. Background technique [0002] Mass spectrometer is an indispensable instrument in today's life science research. It can identify molecules and analyze their structures by measuring the mass of molecules. Tandem mass spectrometry is the most important means when mass spectrometry is used to identify and analyze molecules. Fragment ions are obtained by gas-phase dissociation of molecular ions, and then structural information of molecules can be obtained. There are many mass spectrometers, for example, Matrix-assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) mass spectrometer is a common mass spectrometer with high throughput and high resolution , High sensitivity, widely used in the detection and identification of metabolic small molecules, lipids, sugar ...

Claims

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Application Information

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IPC IPC(8): G01N27/64C07K14/00
CPCG01N27/64G01N33/6851C07K14/001
Inventor 占铃鹏王冠博黄岩谊
Owner SHENZHEN BAY LAB
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