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EupsNPV-Gr strain, biological control agent and application

A nuclear polyhedron, biological control agent technology, applied in the directions of viruses, applications, biocides, etc., can solve the problems of grassland pollution, reduction in the number of natural enemies, destruction of ecological balance, etc., achieve good killing effect, and overcome insecticidal speed. slow effect

Inactive Publication Date: 2022-01-18
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term application of chemical pesticides in grassland pastures will lead to increased resistance of grassland caterpillars, a decrease in the number of natural enemies, and it is easy to cause grassland pollution, destroy ecological balance, and increase pesticide residues.

Method used

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  • EupsNPV-Gr strain, biological control agent and application
  • EupsNPV-Gr strain, biological control agent and application
  • EupsNPV-Gr strain, biological control agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) EupsNPV-Gr virus proliferation experiment:

[0023] The 2nd instar prairie caterpillar larvae were collected in the alpine meadow in Gahai, and fed with Elymus tachycarpa and Bluegrass until the 3rd instar. 100 robust, well-developed 3rd instar prairie caterpillar larvae were selected for multiplication. Dilute the EupsNPV-Gr virus with sterile water to 3×10 6 polyhedral inclusion body (PIB) / mL, sprayed on Elymus tachycarpa and Bluegrass grass with a plastic watering can, dried in the shade, put it into a 21L camellia plastic bucket with a cover, and inserted it into the 3rd instar prairie caterpillar larvae. 25°C, 9:15 in the photoperiod, until all grassland caterpillars died. After feeding poison for 48 hours, replace it with fresh forage. During this period, pay attention to observation every day, and take out the sick and dead grassland caterpillars in time and store them in a refrigerator at 4°C.

[0024] (2) Purification of EupsNPV-Gr virus:

[0025] Take ...

Embodiment 2

[0052] Add EupsNPV-Gr with appropriate amount of sterile water, shake well and count to prepare the required concentration: 1×10 3 PIB / mL, 1×10 4 PIB / mL, 1×10 5 PIB / mL and 1×10 6 There are 4 concentrations of PIB / mL. Draw 20μL of diluent and spread evenly on 1cm 2 The leaf surfaces were dried for bioassay. One group for each concentration, 50 larvae in each group, were placed in sterile containers, the mouth of the bottle was tightly tied with wet gauze, and sterile water was used as a control, and repeated 3 times. After 24 hours of infection, add fresh non-toxic leaves to continue feeding. Regularly observe and record every day, and calculate the corrected mortality rate. The obtained data was analyzed by variance using SPSS software, and linear regression analysis was carried out by using Probit of SPSS software to calculate the half-lethal time (LT50) and half-lethal concentration (Lc50) of different virus concentrations.

[0053] The result is as Figure 5 shown, ...

Embodiment 3

[0055] According to (A) EupsNPV-Gr+Ca 2+ +Bacillus thuringiensis+Azone; (B)EupsNPV-Gr+Ca 2+ +Bacillus thuringiensis+Tinopal LPW+Azone; (C)EupsNPV-Gr+Ca 2+ + Bacillus thuringiensis + Tinopal LPW + titanium dioxide + azone, these three schemes (azone acts as an auxiliary agent for improving adhesion), field bioassay tests were carried out in the alpine grassland in Gannan; the final concentration of EupsNPV-Gr was 10 6 PIB / mL, Ca 2+ Final concentration 0.01%, final concentration of Bacillus thuringiensis 10 3 PIB / mL, the final concentration of Tinopal LPW is 0.5%, the final concentration of titanium dioxide is 0.5%, and the final concentration of azone is 0.5%.

[0056] According to the grassland distribution, terrain and topography, every 10m 2 A community is defined as the scope, and the non-control area with the same conditions is used as the control area. A survey quadrat is randomly selected for each community, and the size of the quadrat is 1m×1m. The control area was...

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Abstract

The invention discloses an EupsNPV-Gr strain, a biological control agent and application of the EupsNPV-Gr strain. The EupsNPV-Gr strain is preserved in China Center for Type Culture Collection on October 19, 2021, and the preservation number of the EupsNPV-Gr is CCTCC NO: V202176. The biological control agent based on the EupsNPV-Gr strain has a very good killing effect on the grassland caterpillars. It is found that the EupsNPV-Gr strain has a good killing effect on the grassland caterpillars. The invention also provides the novel biological control agent at the same time, thus, the technical problems of low insecticidal speed, insufficient insecticidal efficacy, sensitivity to ultraviolet rays and the like when the strain is independently used are solved, and powerful help is provided for biological prevention and control work of the grassland.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a tea caterpillar nuclear polyhedrosis virus Gahai strain EupsNPV-Gr, a biological control agent and application. Background technique [0002] Grassland caterpillars are important pests in my country's plateau pastoral areas. They are also known as red-headed black caterpillars and prairie moths. They belong to the order Lepidoptera and the genus Lepidoptera. There are 15 species of prairie caterpillars in the world, mainly distributed in polar and alpine regions. Grassland caterpillars and grassland rodents and cattle and sheep parasites are listed as the three major grassland pests. Grassland caterpillars are widely distributed (more than 2 million / hm 2 ), high density (usually 200-5 00 heads / m 2 , the highest insect population density reaches 3000 heads / m 2 ), Grassland caterpillars mainly damage Cyperaceae, Gramineae, Fabaceae, Polygonaceae, Rosaceae and othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/40A01N63/23A01N43/90A01N63/27A01P7/04
CPCC12N7/00A01N63/40A01N63/23A01N43/90A01N63/27C12N2710/14121C12N2710/14131C12N2710/14151
Inventor 王金昌靳亮况文东占智高关丽梅陈俊晖
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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