Lactobacillus plantarum capable of degrading tannin and application of lactobacillus plantarum
A Lactobacillus plantarum and plant technology, applied in the field of Lactobacillus, can solve the problems that physical methods are difficult to achieve, the influence of polyethylene glycol is unclear, and the cost of enzymatic hydrolysis is high.
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Embodiment 1
[0074] Example 1: Isolation and identification of Lactobacillus plantarum CAV-M6
[0075] 1. Isolation of Lactobacillus plantarum
[0076] Separation of Lactobacillus plantarum by the dilution plate method: under aseptic conditions, take 10 g of yellow beam wood silage, place it in an Erlenmeyer flask filled with 90 mL of sterile distilled water, and shake it up to obtain a 10-fold diluted sample suspension. According to the 10-fold dilution method, direct serial dilution is enough. Select an appropriate dilution, take 0.1 mL and spread it on the MRS medium plate, incubate in a 30°C incubator for 48 hours, and count the plates. According to the characteristics of colony size, shape and color, typical colonies were picked for Gram staining microscopy and catalase test. All strains that are Gram-positive and catalase-negative can be initially identified as lactic acid bacteria. They are streaked and purified twice on MRS medium, inoculated into MRS solid slant medium, and stor...
Embodiment 2
[0125] Embodiment 2: the application effect of Lactobacillus plantarum CAV-M6 added in the silage
[0126] 1. Preparation of silage (taking woody feed yellow beam wood as an example)
[0127] After mowing at the initial flowering stage, shorten the undried yellow beam leaves to about 2 cm, and mix them evenly to obtain silage raw materials.
[0128] 2. Preparation of Lactobacillus plantarum CAV-M6 bacterial agent
[0129] Inoculate Lactobacillus plantarum CAV-M6 in liquid MRS medium (pH value 6.5), shake culture at 37°C and 150rpm for 24 hours to obtain the bacterial liquid of Lactobacillus plantarum CAV-M6, and in the bacterial liquid of Lactobacillus plantarum CAV-M6 The content of Lactobacillus plantarum CAV-M6 is 10 6 cfu / ml, the Lactobacillus plantarum CAV-M6 bacterial solution is the Lactobacillus plantarum CAV-M6 microbial agent.
[0130] 3. Fermented silage raw materials
[0131] Four experimental groups were set up for each silage raw material, which were the cont...
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