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Egg yolk antibody for resisting antigen excreted and secreted by trichina as well as preparation method and application of egg yolk antibody

A technology for excreting and secreting antigens and yolk antibodies, applied in the field of biomedicine, can solve problems such as difficulties in clinical promotion and application and limitations in clinical use, and achieve the effects of enhancing animal immunity, low cost, and facilitating large-scale industrial production

Pending Publication Date: 2022-03-11
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some people use the weakened trichinella to treat tumor patients, but there are serious side effects, and the clinical use is greatly limited
At present, some studies have been done on polyclonal antibodies and monoclonal antibodies of Trichinella spiralis and tumor cell-associated gene protein antigens. Rat serum and monoclonal antibodies prepared from these related protein antigens have achieved certain anti-tumor effects in animal experiments, but the antibodies used above are all injected, and there are great difficulties in clinical promotion and application.
There is no report on the oral use of poultry yolk antibody against Trichinella spiralis excretory secretory antigen (ESA) for tumor prevention and treatment

Method used

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  • Egg yolk antibody for resisting antigen excreted and secreted by trichina as well as preparation method and application of egg yolk antibody
  • Egg yolk antibody for resisting antigen excreted and secreted by trichina as well as preparation method and application of egg yolk antibody
  • Egg yolk antibody for resisting antigen excreted and secreted by trichina as well as preparation method and application of egg yolk antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Collection and identification of ESA from Trichinella spiralis muscle larvae

[0023] The muscles of mice infected with Trichinella spiralis were digested, and the impurities were removed through 60-mesh and 120-mesh sieves, and the muscle larvae were purified by washing and sedimentation, and the muscle larvae were enriched by centrifugation at 1000 r / min for 5 minutes. Wash repeatedly 5 times with 1×PBS containing 1% penicillin and streptomycin, and collect worms by centrifugation. Repeatedly washed 3 times with 1×PBS, collected worms by centrifugation, added 15ml PBS and incubated at 37°C for 72h. Collect the culture solution and centrifuge to get the supernatant, which is the muscle larvae ESA. Use a filter to remove impurities. Finally, use a 3KDa ultrafiltration tube at 4°C, 6500r / min to ultrafilter to 5ml, measure the concentration by BCA method, and store at -20°C. Take 50 μl of ESA sample preparation and perform SDS-PAGE and Coomassie Brilliant Blue staining, ...

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Abstract

The invention provides an anti-trichina excretion secretion antigen (ESA) chicken egg yolk antibody, and also discloses a medical application of the egg yolk antibody in tumor resistance. The composition has the characteristics of animal immunity enhancement, no toxicity and the like, can be used as an oral preparation for treating tumors such as lung cancer, and can be prepared into oral liquid and the like as food for preventing tumors; the egg yolk antibody for poultry such as chicken and the like can be orally taken, is convenient to use, low in cost, non-toxic, safe and convenient for large-scale industrialized production.

Description

Technical field: [0001] The present invention relates to an anti-trichinella secretory antigen (Excretory secretory antigen, ESA) egg yolk antibody, especially discloses anti-trichinella spiralis excretory secretory antigen (ESA) chicken egg yolk antibody and its preparation method; The medical application in antitumor belongs to the technical field of biomedicine. Background technique: [0002] Trichinella ( Trichinella spiralis ) is a zoonotic parasite that seriously endangers human health. Its adults and larvae live in the intestinal tract and muscle tissue of mammals, respectively. With the in-depth research on Trichinella spiralis, people found that besides causing trichinellosis, the infection of Trichinella spiralis can improve the anti-tumor ability of the host body. Some people use the weakened trichinella to treat tumor patients, but there are serious side effects, and the clinical use is greatly limited. At present, some studies have been done on polyclonal ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/02A61K39/395A61P35/00
CPCC07K16/18C07K16/02A61P35/00C07K2317/11A61K2039/505
Inventor 张西臣岳涛涛张楠李建华宫鹏涛王瑜
Owner JILIN UNIV
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