Method for establishing acute renal failure model of dog

Abstract

The invention belongs to the technical field of acute renal failure model construction, and discloses a method for establishing an acute renal failure model of a dog, which comprises the following steps of: determining and grouping test animals; determining instruments and reagents; collecting a sample; determining indexes; and statistical analysis. According to the method for establishing the canine acute renal failure model, adenine is adopted to induce establishment of the canine acute renal failure model, whether the model is successfully established or not is judged through detection of indexes such as blood and urine and pathological changes of kidney tissue, and then the optimal dose of adenine induced canine acute renal failure is determined. A comprehensive result shows that an acute renal failure model can be successfully established by adding 75mg / kg.d adenine to a dog for feeding for 15 days, that is, the modeling dosage used by a 75mg adenine model group is proper, an animal can reach an ideal modeling state of acute renal failure after modeling for 15 days, and meanwhile, extra injury caused by operation and the like to a test animal can be avoided, and acute death of the animal can be avoided.

Description

technical field [0001] The invention belongs to the technical field of acute renal failure model construction, in particular to a method for establishing a canine acute renal failure model. Background technique [0002] At present, in the basic and clinical research of acute renal failure, in order to deeply understand the formation process of acute renal failure and explore the formation mechanism of acute renal failure, it is particularly important to establish experimental animal models of acute renal failure. At present, most of the experimental animal models of acute renal failure used in research at home and abroad are established on experimental animals such as rats or rabbits, and the purpose of establishing animal models of acute renal failure is achieved by means of physical or chemical drugs. [0003] There are two main physical methods, one is to cause renal tissue ischemia through physical and mechanical methods, causing the degeneration and necrosis of renal tu...

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Problems solved by technology

[0007] The significance of solving the above problems and defects is: most of the experimental animals for making acute renal failure model animals reported at home and abroad are rats and rabbits, and seldom can be consulted about making acute renal failure model dogs. The method of physical or chemical drugs achieves the purpose of establishing animal models of acute renal failure

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