A kind of construction method of animal model of diabetic nephropathy

A technology for diabetic nephropathy and animal model, applied in the field of pharmacology, can solve the problem that the pathological and physiological changes of diabetic nephropathy cannot be fully reflected, and achieve the effects of short modeling time, enhanced practicability and high success rate

Active Publication Date: 2019-01-29
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

[0005] At present, there are few research models on diabetic nephropathy, including spontaneous or genetic animal models, based on chemical drug induction, high-fat and high-sugar feed induction, surgical resection of pancreas and transgenic animal models, but generally based on diabetic animal models It cannot fully reflect the pathological and physiological changes in the process of diabetic nephropathy

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  • A kind of construction method of animal model of diabetic nephropathy
  • A kind of construction method of animal model of diabetic nephropathy
  • A kind of construction method of animal model of diabetic nephropathy

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Embodiment Construction

[0022] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0023] In order to truly restore the influence of environmental pollution on the occurrence of diabetic nephropathy as much as possible, the present invention provides a method for constructing an animal model of diabetic nephropathy by using a method of chronic cadmium poisoning. Animals in the present invention include but are not limited to: mice, rats, beagle dogs, rhesus monkeys, pigs, cows and other animals that are closely related to scientific experiments and people's lives (the following takes mice as an example).

[0024] 1. Animal preparation

[0025] Thirty adult Kunming male mice, weighing 20g±2g, had free access to food and water, and their living rhythm followed the circadian rhythm.

[0026] 2. Animal grouping and model construction

[0027] After one week of adaptive feeding, the mice were randomly divided into a control group (Cont...

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Abstract

The invention provides a method for constructing a diabetic nephropathy animal model.The influence of environmental pollution on disease occurrence is simulated, cadmium chloride serves as an inductor of the diabetic nephropathy animal model, administration is mainly achieved through intraperitoneal injection, high glucose and high fat are combined for feeding for 8 weeks, the physiological and biochemical index detection result shows that cadmium chloride can cause rise of blood glucose and increase of the NAG content, and pathological examinations meet the pathological change of diabetic nephropathy.The method has the advantages of being easy to operate, economical, high in model making success rate and the like, the pathogenic process of diabetic nephropathy can be simulated, and the problem that an existing diabetic nephropathy animal model is single is solved.

Description

technical field [0001] The invention belongs to the field of pharmacology, and relates to a method for constructing an animal model of diabetic nephropathy. Background technique [0002] Diabetes (diabetes mellitus, DM) is a serious global public health problem. The prevalence of diabetes in my country has a "blowout" trend. China has replaced India as the largest country with diabetes. The prevalence of diabetes is 9.7%, about 9,000 million Chinese suffer from diabetes. At present, my country's annual DM medical expenditure reaches 20 billion yuan, accounting for 4.38% of the total consumption of national health resources, which seriously hinders my country's economic development. Its harmfulness is mainly manifested in various chronic complications. It is not only the main cause of death and disability of diabetic patients, but also causes a heavy economic burden. [0003] Diabetic nephropathy (diabetic nephropathy, DN) is a systemic microvascular complication of diabetes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A61K33/24A23K20/158A23K20/163
CPCA01K67/02A61K33/24
Inventor 龚频皮思慧王兰杨文娟常相娜
Owner SHAANXI UNIV OF SCI & TECH
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