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Taiwan sedum lineare tissue culture and rapid propagation method

A technology of tissue culture and severabes, applied in the direction of culture medium, horticultural methods, botanical equipment and methods, etc., can solve the problems of low survival rate of cutting propagation, low seeding germination rate, easy to spread diseases, etc. Difficulty, increase proliferation efficiency, and shorten the effect of proliferation time

Active Publication Date: 2022-03-22
杭州萧山技师学院
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AI Technical Summary

Problems solved by technology

However, the seeds of S. taiwanensis are small, the germination rate of sowing is low, the survival rate of cutting propagation is not high, and it is easy to spread diseases, which seriously restricts its application in the garden market.

Method used

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Experimental program
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Effect test

Embodiment

[0015] A method for tissue culture and rapid propagation of S. taiwanensis, comprising the following steps:

[0016] S1. Material selection and pretreatment: Select a plant that grows robustly and has no pests and diseases as the mother plant. One week before sampling, use 50% thiophanate-methyl 1000 times solution to spray the sampled mother plant the next day to reduce the amount of blood in the mother plant. Carried endophytes, thereby optimizing the disinfection effect of explants. When sampling, cut off the young stems at the top, remove the residual leaves, and wrap them in a damp cloth for later use.

[0017] S2. Disinfection treatment of explants: soak the retrieved young stem segments in water containing 10% washing powder for 5 minutes, and rub gently during the process to remove foreign matter on the stem segments. Then put the explants into a 1L glass beaker, rinse them under running water for 30 minutes, then soak them in 1000-fold carbendazim solution for 10 min...

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Abstract

According to the Taiwan sedum lineare tissue culture and rapid propagation method, a preventive sterilization and disinfection means before sampling is adopted, so that the bacterium carrying rate of an explant is reduced by about 50%, and the disinfection effect of the explant is greatly improved; all used reagents are low-cost reagents, the rooting culture medium does not need to be added with any plant hormone, the same rooting effect as other tissue culture technologies can be achieved, the production cost is reduced, and meanwhile the preparation difficulty is reduced; according to the culture medium formula for inoculating the explant, the explant can directly regenerate a new bud without a callus stage, so that the proliferation time is shortened, the proliferation efficiency is improved, and the time cost is greatly reduced for industrialized production.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of S. taiwanensis. Background technique [0002] Sedum formosanum N.E.Br. (scientific name: Sedum formosanum N.E.Br.) is a perennial herbaceous plant belonging to the genus Sedum formosanum in the family Crassulaceae. This species is evergreen in all seasons and has a large crown. It is suitable for flower mirrors, ground covers, and potted plants. The flowers and leaves are beautiful, and the ornamental effect is excellent. However, the seeds of S. taiwanensis are small, the germination rate of sowing is low, the survival rate of cutting propagation is not high, and diseases are easy to spread, which seriously restricts its application in the garden market. It is beneficial to plant tissue culture technology to carry out the breeding of S. taiwanensis, which can not only greatly increase the reproduction coefficient, but also replace resource gardens that occupy a large amoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/15A01G22/00
CPCA01H4/002A01H4/008A01G24/28A01G24/15A01G22/00
Inventor 詹菁马广莹何林夫何颖丽李四明陈聪玮
Owner 杭州萧山技师学院
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