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Anti-aging composition for increasing dermal collagen protein mass and maintaining fibroblast morphology and application of anti-aging composition

A composition and total quality technology, which is applied in the field of daily chemical industry, can solve the problems of not mentioning the shape of fibroblasts, lack of collagen, and not simultaneously repairing the quality of collagen in the dermis and the shape of fibroblasts

Pending Publication Date: 2022-03-25
SHANGHAI PECHOIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these patents, there is no mention of the quality of the newly synthesized collagen, nor the integrity of the fibroblast morphology
[0005] At present, there are many technologies to promote collagen synthesis, but there is a lack of technologies to promote complete, orderly and high-quality collagen synthesis; there is also a lack of technologies for the protection of fibroblast morphology, especially under UV stress conditions. technology; there is no technology to repair the collagen quality and fibroblast morphology of the dermis at the same time, so as to achieve the youthfulness of the dermis; in addition, the field expects to be comparable to retinol in terms of efficacy, but it does not cause skin irritation and does not Combinations that disrupt the skin barrier

Method used

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  • Anti-aging composition for increasing dermal collagen protein mass and maintaining fibroblast morphology and application of anti-aging composition
  • Anti-aging composition for increasing dermal collagen protein mass and maintaining fibroblast morphology and application of anti-aging composition
  • Anti-aging composition for increasing dermal collagen protein mass and maintaining fibroblast morphology and application of anti-aging composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] 1. Reconstruction of the dermis model + drug application

[0070] Human primary fibroblasts (dermal fibroblasts from an 18-year-old female) were placed in a matrix (Mimedisc TM ) for 35 days, and the medium was replaced every two days for cultivating the reconstructed dermis model; on the 36th day, retinol (10 μM) dissolved in dimethyl sulfoxide (DMSO) or dissolved in the medium ( That is, the above-mentioned matrix for culturing primary fibroblasts) of the composition of the present invention (based on the total weight of the culture medium, containing 0.318% of the composition) was respectively applied to the reconstructed dermis model for 8 consecutive days.

[0071] After the composition of the present invention is dissolved in the medium, the concentration of component (a) is 0.15%, the concentration of component (b) is 0.015%, and the concentration of component (c) is 0.15% based on the total weight of the medium. %, the concentration of component (d) is 0.003%. ...

Embodiment 2

[0081] 1. Experimental materials

[0082] Test system: The skin model used in this test is a 3D full-thickness skin model Produced by Guangdong Boxi Biotechnology Co., Ltd., the model batch numbers are: F200701 and F200801.

[0083] Main reagents: FSK4 culture medium (special culture medium for 3D full-thickness skin model; Guangdong Boxi), fetal bovine serum (Sijiqing), PBS (Boster), horseradish catalase DAB color development kit (Shenggong) , Paraformaldehyde (Chinese medicine), xylene (Chinese medicine), absolute ethanol (Chinese medicine), hematoxylin (Biyuntian), eosin (Biyuntian).

[0084] Main equipment: CO 2 Incubator (Thermo), ultra-clean bench (Sujing Antai), UVA lamp (Philips), UVB lamp (Philips), microplate reader (BioTek), micro-oscillator (Qilin Bell), upright microscope ( Olympus).

[0085] Test recipe:

[0086]

[0087] 2. Skin model processing

[0088] (a) Model grouping: transfer the model to a 6-well plate, and absorb 0.9 mL / well of FSK4 (containin...

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Abstract

The invention discloses a composition as well as a preparation method and application thereof. The composition comprises a component (a), a component (b), a component (c) and a component (d), the component (a) is a soybean hydrolysate, the component (b) is a rambutan leaf extract, the component (c) is a kangaroo claw extract, and the component (d) contains a false leaf root extract and escin.

Description

technical field [0001] The invention relates to the field of daily chemical industry, in particular to an anti-aging combination and application for improving the collagen quality of the dermis and maintaining the shape of fibroblasts. Background technique [0002] Skin aging is closely related to the extracellular matrix of the dermis. Collagen fibers are an important component of the extracellular matrix, accounting for 75% of the dry weight of the skin, and provide the skin with toughness and elasticity. The change of extracellular matrix is ​​an important reason for skin aging. The thickness of collagen fibers in old skin becomes uneven and the structure becomes more disordered. At the same time, the integrity of collagen itself is also destroyed by matrix metalloproteinases. Fibroblasts reside in a network formed by the extracellular matrix in the dermis. Fibroblasts are also cells responsible for the synthesis of extracellular matrix. Young fibroblasts are attached ...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/9794A61K8/63A61Q19/08A61Q19/10
CPCA61K8/9789A61K8/9794A61K8/63A61Q19/08A61Q19/10
Inventor 左锦辉刘炎华陈兴陈斌
Owner SHANGHAI PECHOIN BIOTECH CO LTD
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