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Colon cancer biomarker and application thereof

A biomarker and colon cancer technology, applied in biomaterial analysis, measuring devices, instruments, etc., can solve the problems that the diagnostic value of colon cancer has not yet been studied.

Pending Publication Date: 2022-03-25
THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biological role of ENTPD2 in the colon cancer microenvironment and its diagnostic value in colon cancer have not been investigated

Method used

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  • Colon cancer biomarker and application thereof
  • Colon cancer biomarker and application thereof
  • Colon cancer biomarker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Relationship between serum exosome ENTPD2 (Gene ID: 954) and colon cancer

[0035] 1. Experimental method

[0036] 1. Immunohistochemistry

[0037] (1) Bake the colon cancer tissue chip (Shanghai Xinchao Biotechnology Co., Ltd., China, article number #HColA180Su21) at 65°C for 2 hours;

[0038] (2) Dewaxing: soak the slides in xylene I for 5 minutes, xylene II for 5 minutes, xylene III for 5 minutes, ethanol I for 5 minutes, ethanol II for 5 minutes, 95% Soak in ethanol for 5 minutes, 85% ethanol for 5 minutes, 75% ethanol for 5 minutes, ddH 2 Soak in O I for 3min, ddH 2 Soak in O II for 3 minutes;

[0039] (3) Block peroxidase: soak in 3% hydrogen peroxide for 10 minutes, wash with PBS for 5 minutes;

[0040] (4) High-pressure antigen restoration; add EDTA repair solution (Bioworld, USA, product number BD5033) into the pressure cooker, put the glass slide into the pot, heat at 800W for 20 minutes, and let it cool naturally;

[0041] (5) Wash the glass s...

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Abstract

The invention belongs to the technical field of tumor molecular diagnosis, and particularly relates to a colon cancer biomarker and application thereof.The research finds that an exosome of a colon cancer patient can stably express ENTPD2, the expression level of the exosome is obviously higher than that of a healthy person, and the exosome ENTPD2 with the high level is closely related to TNM staging and tumor infiltration depth of the patient; therefore, the serum exosome ENTPD2 has obvious advantages in colon cancer diagnosis, is expected to become a novel biological marker for colon cancer screening, individualized treatment and prognosis monitoring, and provides a new direction for colon cancer treatment. The invention finds that the ENTPD2 has obvious high expression in colon cancer tissues for the first time, the expression level of the ENTPD2 is closely related to the prognosis of patients, and the ENTPD2 is proved to be beneficial to the early diagnosis and prognosis judgment of the colon cancer patients.

Description

technical field [0001] The invention belongs to the technical field of tumor molecular diagnosis, and in particular relates to a colon cancer biomarker and its application. Background technique [0002] Colorectal cancer (CRC) is a common malignant tumor of the digestive tract, and the overall incidence of CRC ranks third in the world. The onset of CRC is hidden, and most patients are already in the middle and advanced stages when they are discovered, so most patients lose the best opportunity for treatment. Therefore, an in-depth study of the occurrence and development mechanism of CRC and the screening of valuable diagnostic markers will help provide reference for clinical decision-making in the links of prevention, diagnosis and treatment, and prognosis. [0003] Precision medicine plays an important role in tumor diagnosis and treatment. Among them, liquid biopsy, as an alternative technology to traditional biopsy, obtains tumor information through non-invasive samplin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573
CPCG01N33/57419G01N33/57488G01N33/573G01N2333/914
Inventor 刘焕亮石梦辰杨湘玲贾会真韦丽丽张瀞丹田宇
Owner THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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