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Long-chain non-coding RNA (Ribonucleic Acid) gene for increasing rice yield and application thereof

A technology of rice and genes, applied in the field of lncRNA

Active Publication Date: 2022-04-19
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the lncRNA that regulates the differential development of primary and secondary caryopsis in rice

Method used

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  • Long-chain non-coding RNA (Ribonucleic Acid) gene for increasing rice yield and application thereof
  • Long-chain non-coding RNA (Ribonucleic Acid) gene for increasing rice yield and application thereof
  • Long-chain non-coding RNA (Ribonucleic Acid) gene for increasing rice yield and application thereof

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Experimental program
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Embodiment Construction

[0021] The following experimental methods, unless otherwise specified, are conventional methods. The reagents used in the following experimental methods were purchased from conventional biochemical reagent companies unless otherwise specified.

[0022] Plant material: Oryza sativa L. subspecies japonicacv. Zhonghua11

[0023] 1. Rice Cultivation Method

[0024] The rice cultivation site is the rice field of the Biological Garden of the School of Life Sciences, Beijing Normal University. In mid-April, a sufficient amount of 1% carbendazim (commercially available) solution was prepared, and rice seeds (with glumes) were soaked overnight. The next day, rinse with plenty of water to remove the carbendazim solution, and change to soaking in water, changing the water twice a day in the morning and evening until the germ grows. Spread the germinated seeds evenly in the seedling tray, and spread a thin layer of covering soil. After fully watering, transfer the seedling tray to an ...

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Abstract

The invention discloses a long-chain non-coding RNA (Ribonucleic Acid) gene for increasing the yield of rice and application of the long-chain non-coding RNA gene. The rice long-chain non-coding RNA (lncRNA) gene is named as XLOC050158, is highly expressed 5 days after primary caryopses bloom, is highly expressed 12 days after secondary caryopses bloom, and belongs to typical rice caryopses medium-development delayed expression lncRNA. Through construction of XLOC050158 overexpression rice and phenotype observation, it is found that the grain weight of secondary caryopses of the overexpression rice is increased and is close to the grain weight of primary caryopses, and the plant height, the spike length, the total spikelet number of a single spike, the primary spikelet number and the secondary spikelet number of the overexpression rice do not change obviously. The invention provides important scientific basis and technical support for obtaining high-yield rice.

Description

technical field [0001] The present invention relates to a long-chain non-coding RNA (lncRNA) that improves rice yield components and significantly increases yield, and particularly relates to an lncRNA that regulates the developmental state of rice secondary caryopsis to increase its grain weight and its application. Background technique [0002] Rice is an important food crop, accounting for more than one-third of my country's total grain output. Therefore, increasing rice production is of great strategic significance to ensure my country's food security and sustainable development. The expansion of rice storage capacity (yield potential) is an important prerequisite for its super-high yield. In the past ten years, many research institutions at home and abroad, such as my country, Japan, and the International Rice Research Institute, have successfully bred many new short-stem panicle heavy-duty and large-grain new plant types of rice, and their storage capacity has been gr...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/84A01H5/08A01H6/46
CPCC12N15/113C12N15/8261C12N2310/10Y02A40/146
Inventor 王英典韩生成王晗梦
Owner BEIJING NORMAL UNIVERSITY
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