Nucleic acid extractor magnetic bead transfer system supporting variable solution volume and nucleic acid extractor

Abstract

According to the nucleic acid extraction instrument magnetic bead transfer system supporting the variable solution volume and the nucleic acid extraction instrument, by improving a connecting structure and a driving structure of a magnetic rod frame, a magnetic rod sleeve frame and a pore plate tray, the displacement freedom degree of the nucleic acid extraction instrument for operation can be increased; therefore, the nucleic acid extraction instrument can realize more complex displacement modes except synchronous column displacement, particularly, magnetic beads of a plurality of hole sites are combined into the same hole site, the limitation of the inherent hole site volume on a hole plate is exceeded, and the large-range adjustment change of different reagent volumes in the operation process is supported. By applying the magnetic bead transfer system of the nucleic acid extraction instrument with the variable solution volume, the nucleic acid extraction instrument can simultaneously support high-flux nucleic acid extraction operation requirements of various different samples.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid extractor magnetic bead transfer system and a nucleic acid extractor supporting variable solution volumes. Background technique [0002] In recent years, nucleic acid extraction technology has developed rapidly, and various nucleic acid extraction instruments have emerged in an endless stream. Biotechnology with nucleic acid extraction technology as the research object mainly focuses on the extraction, amplification, and sequencing of nucleic acids. Among them, nucleic acid extraction technology is highly regarded as the basis of all other technologies. The pros and cons of nucleic acid extraction technology directly determine the operational efficiency of other subsequent steps and the final quality of the experiment. [0003] Among the nucleic acid extraction methods, the research on the magnetic bead method is particularly prominent. The extrac...

AI Technical Summary

Problems solved by technology

However, for the existing nucleic acid extractors, due to the limitation of the degree of freedom of movement, the transfer of magnetic beads can only be carried out synchronously in the form of columns, and the adjustment of the volume of reagents in different steps can only be pre-packaged in the holes of the same volume. The amount of reagent added to each hole is slightly different, and the adjustable range is very small, which cannot achieve ideal volume changes of different reagents.

[0004] On the other hand, for different types of nucleic acid samples, such as FFPE samples and cfDNA samples, although the magnetic bead method can be used for nucleic acid extraction, due to factors such as the characteristics of the sample itself, the difference between the wells of the wells of the well plate used for FFPE extraction The same volume between different wells, different reagent volumes but the difference is small (900ul-100ul), while cfDNA extraction operates on wells of different sizes, and the volume difference between the wells is large, and the volume of the liquid added also varies greatly ( 12ml—60ul), it is necessary to use different volumes of nucleic acid extraction reagents to obtain a nucleic acid solution suitable for subsequent detection

Therefore, in the prior art, different nucleic acid extractors must be used for different nucleic acid sample types to perform the normal extraction operation, and there is no universality between them, and the throughput provided by the existing nucleic acid analyzers is relatively small

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