Artificial seawater domestication method of prorocentrum capable of producing diarrheic shellfish poison
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A technology for diarrheal shellfish poisoning and artificial seawater, which is applied in the directions of microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc. Improve the effect of precise control and reduce culture cost
Pending Publication Date: 2022-05-06
青岛普瑞邦生物工程有限公司
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However, there is currently no report on an effective method for artificially domesticating Prorocentrum
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Embodiment 1
[0038] Embodiment 1 The artificial seawater domestication of Prorocentrum lima
[0039] 1. Domestication method:
[0040] (1) Configuration of mixed seawater culture solution:
[0041] Set up the experimental group 1 and the experimental group 2, mix the sterilized artificial seawater with the natural seawater according to the ratio in the following table, and prepare mixed seawater culture solutions at all levels.
[0042] Table 3 The proportion setting of artificial seawater and natural seawater in the mixed seawater culture solution
[0043]
[0044]
[0045] The specific operation is as follows:
[0046] Now the artificial seawater, natural seawater and f / 2 culture solution mother solution (without f / 2 vitamin mother solution) are sterilized respectively under the condition of 110°C for 20 minutes.
[0047] After mixing the above-mentioned artificial seawater and natural seawater in proportion to obtain the mixed seawater culture solution, add the aforementioned f / 2...
Embodiment 2
[0073] Analysis of the growth performance of Prorocentrum lima after embodiment 2 domestication
[0074] 1. Experimental materials:
[0075] The algal species is Prorocentrum lima (CCMA-Pl-071), cultured to the exponential phase as the seed solution, the cell density is about 8000cells / mL.
[0076] 2. Preparation of culture medium:
[0077] (1) Natural seawater is taken from the sea area near Shilaoren, Qingdao. After a week of dark precipitation, it is used after suction filtration with a 0.22um water filter membrane.
[0078] (2) Artificial seawater: with embodiment 1.
[0079] (3) The formulation and preparation method of the f / 2 culture solution mother solution are well-known operations by those skilled in the art, see the above content.
[0080] 3. Experimental group settings:
[0081] The natural seawater group, the domestication culture group and the artificial seawater group were respectively set up for control experiments, and three parallel samples were set for e...
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Abstract
The invention discloses an artificial seawater domestication method of prorocentrum capable of producing diarrheal shellfish poison, which comprises the following steps: S1, inoculating a prorocentrum seed solution into a primary mixed seawater culture solution, fully shaking up the system, and carrying out standing culture; s2, absorbing the algae liquid obtained in the previous step, inoculating the algae liquid into a secondary mixed seawater culture solution, and carrying out standing culture; s3, transferring the obtained algae liquid into a third-stage mixed seawater culture solution according to the method in the previous step, culturing for 15-25 days, then transferring the algae liquid into a next-stage mixed seawater culture solution for culturing, and repeating the step; and S4, finally, inoculating the obtained algae liquid into artificial seawater to complete domestication. The mixed seawater culture solution mainly comprises artificial seawater and natural seawater, and the volume ratio of the artificial seawater to the natural seawater is gradually increased in a gradient manner along with the increase of the transfer times. The prorocentrum subjected to artificial seawater domestication treatment can realize complete artificial seawater culture, so that the culture cost of the prorocentrum species is greatly reduced.
Description
technical field [0001] The invention relates to the technical field of artificial seawater domestication of algae, in particular to a method for domestication of Prorocentrum artificial seawater producing diarrhea shellfish poisoning. Background technique [0002] Prorocentrum lima is a marine benthic dinoflagellate that can produce diarrheal shellfish poisoning DSP , widely distributed in tropical and temperate coastal waters. Diarrhetic shellfish poison (DSP) is one of the important toxins in red tide toxins. It is named because the symptoms of the poisoning are mainly diarrhea. Diarrheal shellfish poisoning mainly includes okadaic acid (OA), dinophysistoxin-1 (DTX-1), DTX-2 and DTX-3. OA is a highly selective and potent inhibitor of serine / threonine protein phosphatases (PP1 and PP2A), which can modulate intracellular protein metabolism and have a wide range of effects on eukaryotic cellular processes. extremely effective tool. Due to its potential physiological activ...
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