Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene
A plant and protein technology, applied in the field of plant endogenous genes and their proteins, can solve the problems of environmental and personal health threats, chemical pesticides irritating odor, etc., and achieve the effect of inhibiting feeding and improving plant insect resistance.
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[0025] The above content of the present invention will be further described in detail in the form of preferred implementation examples below, but it does not constitute a limitation to the present invention.
[0026] Unless otherwise specified, the reagents in the examples of the present invention can be purchased through commercial channels.
[0027] Order Arabidopsis Col-0 (wild type) and cznky2020002 T-DNA insertion mutant cznky2020002 (seed number: SALK_023552) on the official website of ABRC (https: / / abrc.osu.edu / ). figure 1 The gene structure of cznky2020002 is shown. Such as figure 1 As shown, in cznky2020002, the T-DNA was inserted at the sixth exon. Wherein, the nucleic acid sequence of cznky2020002 is shown in SEQ ID No.1, and the amino acid sequence of its protein is shown in SEQ ID No.2.
[0028] The seeds of the Arabidopsis mutant cznky2020002 were spread on MS medium, and when the Arabidopsis plants grew to 4-6 leaves, they were transplanted to vermiculite for...
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