Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene
A plant and protein technology, applied in the field of plant endogenous genes and their proteins, can solve the problems of environmental and personal health threats, chemical pesticides irritating odor, etc., and achieve the effect of inhibiting feeding and improving plant insect resistance.
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[0025] The above contents of the present invention will be further explained in detail in the form of the following preferred embodiments, but they do not constitute the limitation of the present invention.
[0026] Unless otherwise specified, the reagents in the embodiments of the present invention can be purchased commercially.
[0027] Order Arabidopsis Col-0 (wild type) and cznky2020002T-DNA insertion mutant cznky2020002 (seed number: SALK_023552) in ABRC(https: / / abrc.osu.edu / ) official website. Figure 1 The gene structure of cznky2020002 is shown. such as Figure 1 As shown, in cznky2020002, T-DNA is inserted at exon 6. Thereinto, the nucleic acid sequence of cznky2020002 is shown in SEQ ID No.1, and the amino acid sequence of its protein is shown in seq id No.2.
[0028] Seed spots of Arabidopsis mutant cznky2020002 were spread on MS medium, and when Arabidopsis plants grew to 4-6 leaves, they were transplanted to vermiculite for growth (24℃; Light: dark = =16h:8h), and after...
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