Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene

A plant and protein technology, applied in the field of plant endogenous genes and their proteins, can solve the problems of environmental and personal health threats, chemical pesticides irritating odor, etc., and achieve the effect of inhibiting feeding and improving plant insect resistance.

Pending Publication Date: 2022-05-06
CANGZHOU ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the control of crop pests mainly relies on chemical pesticides, but chemical pesticides have a pungent smell and pose a serious threat

Method used

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  • Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene
  • Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene
  • Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene

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[0025] The above contents of the present invention will be further explained in detail in the form of the following preferred embodiments, but they do not constitute the limitation of the present invention.

[0026] Unless otherwise specified, the reagents in the embodiments of the present invention can be purchased commercially.

[0027] Order Arabidopsis Col-0 (wild type) and cznky2020002T-DNA insertion mutant cznky2020002 (seed number: SALK_023552) in ABRC(https: / / abrc.osu.edu / ) official website. Figure 1 The gene structure of cznky2020002 is shown. such as Figure 1 As shown, in cznky2020002, T-DNA is inserted at exon 6. Thereinto, the nucleic acid sequence of cznky2020002 is shown in SEQ ID No.1, and the amino acid sequence of its protein is shown in seq id No.2.

[0028] Seed spots of Arabidopsis mutant cznky2020002 were spread on MS medium, and when Arabidopsis plants grew to 4-6 leaves, they were transplanted to vermiculite for growth (24℃; Light: dark = =16h:8h), and after...

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Abstract

The invention provides a plant endogenous gene influencing lepidoptera insect feeding and a protein thereof. The plant endogenous protein can influence the plant feeding amount of insects, and the amino acid sequence of the protein is as shown in SEQ ID No.2.

Description

technical field [0001] The invention relates to a plant endogenous gene and its protein that affect the feeding of lepidopteran insects, especially the homozygous plants that lack or silence the gene can acquire resistance to lepidopteran insects. Background technique [0002] In agricultural production, pests are one of the important reasons affecting crop yield and quality, and cause huge economic losses to farmers every year. Lepidoptera includes two classes of insects, moths and butterflies, belonging to the subclass Pteroptera and holometabolism. This order is the second largest order after Coleoptera in Insecta, with a wide range of distribution and the most abundant species in the tropics. Most types of larvae damage all kinds of cultivated plants, and the larger ones often eat up the leaves or drill into the branches. Smaller ones tend to curl leaves, adorn leaves, form sheaths, spin silk and form nets, or drill into plant tissues to feed and cause damage. Adults ...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/29C07K14/415A01H5/00A01H6/20
CPCC12N15/8286C12N15/8218C07K14/415
Inventor 张悦宋素胜刘春琴周璐璐王庆雷寇俊凤吴娱冯晓洁刘福顺
Owner CANGZHOU ACAD OF AGRI & FORESTRY SCI
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