Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene

A plant and protein technology, applied in the field of plant endogenous genes and their proteins, can solve the problems of chemical pesticides irritating odor, environmental and personal health threats, and achieve the effect of improving plant insect resistance and inhibiting feeding

Pending Publication Date: 2022-05-24
CANGZHOU ACAD OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the control of crop pests mainly relies on chemical pesticides, but chemical pesticides have a pungent smell and pose a serious threat

Method used

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  • Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene
  • Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene
  • Plant endogenous gene influencing feeding of lepidoptera insects and protein of plant endogenous gene

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Embodiment Construction

[0025] The above content of the present invention will be further described in detail below in the form of preferred embodiments, but it does not constitute a limitation to the present invention.

[0026] Unless otherwise specified, the reagents in the examples of the present invention can be purchased through commercial channels.

[0027] Order Arabidopsis Col-0 (wild type), cznky2020001T-DNA insertion mutant cznky2020001-1 (seed number: SALK_018601) and cznky2020001T-DNA insertion mutant from ABRC (https: / / abrc.osu.edu / ) official website Body cznky2020001-2 (seed number: SALK_015611). figure 1 The gene structure of cznky2020001 is shown. like figure 1 As shown, in cznky2020001-1, the T-DNA was inserted at exon 8; in cznky2020001-2, the T-DNA was inserted at exon 12. Among them, the nucleic acid sequence of cznky2020001 is shown in SEQ ID No.1, and the amino acid sequence of its protein is shown in SEQ ID No.2.

[0028] The seeds of Arabidopsis mutants cznky2020001-1 and ...

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Abstract

The invention provides a plant endogenous gene influencing lepidoptera insect feeding and a protein thereof. The plant endogenous protein can influence the plant feeding amount of insects, and the amino acid sequence of the protein is as shown in SEQ ID No.2.

Description

technical field [0001] The present invention relates to a plant endogenous gene and its protein that affect the feeding of lepidopteran insects, in particular, the homozygous plant after deletion or silencing of the gene can acquire resistance to lepidopteran insects. Background technique [0002] In agricultural production, pests are one of the important reasons affecting crop yield and quality, causing huge economic losses to farmers every year. Lepidoptera includes two types of insects, moths and butterflies. This order is the second largest order in the class of insects after Coleoptera, with a wide distribution range, with the most abundant tropical species. Most species of larvae damage all kinds of cultivated plants, and the larger ones often eat up leaves or bore through branches. Smaller ones tend to roll leaves, tie leaves, knot sheaths, spin silk and net or drill into plant tissues to feed. Adults mostly use nectar as supplementary nutrition, or the mouthparts ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12Q1/6895C12N15/11
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156Y02A40/146
Inventor 张悦刘春琴王庆雷赖德强宋素胜寇俊凤冯晓洁刘福顺吴娱
Owner CANGZHOU ACAD OF AGRI & FORESTRY SCI
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