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IGFBP7 muscle tissue specific knockout mouse animal model and construction method thereof

A technique of muscle tissue, construction methods, applied in the field of genetics and biology, which can solve problems such as poorly understood functions

Pending Publication Date: 2022-05-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the role of IGFBP7 in muscle biology is poorly understood

Method used

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  • IGFBP7 muscle tissue specific knockout mouse animal model and construction method thereof
  • IGFBP7 muscle tissue specific knockout mouse animal model and construction method thereof
  • IGFBP7 muscle tissue specific knockout mouse animal model and construction method thereof

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Embodiment Construction

[0036] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. The following examples are intended to illustrate the present invention, but not to limit the scope of the present invention. The third exon in the present invention is the third exon of the C57BL / 6N mouse IGFBP7 gene.

[0037] The construction method of the IGFBP7 muscle tissue-specific knockout mouse animal model of the present invention is specifically implemented according to the following steps:

[0038] 1. The design of the knockout mouse targeting vector, the specific steps are as follows:

[0039] According to the C57BL / 6N mouse IGFBP7 gene (NCBI reference sequence: NC_000071.6) published by NCBI, the gene has 6 exons, of which the ATG start codon is located in exon 1, and the TAA stop codon is located in exon Sub 6. The exon 3 shared by the two transcripts NM_008048.3 (encoding 282 amino acids) and NM_001159518.1 (encoding 313 amino aci...

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Abstract

The invention relates to an IGFBP7 muscle tissue specific knockout mouse animal model and a construction method thereof. The mouse model constructed by the method is used for exploring important functions and molecular mechanisms of the IGFBP7 gene in mouse muscle development. A third exon in the IGFBP7 gene is knocked out from the mouse model, an F0 chimeric mouse is constructed through construction of a targeting vector and ES targeting in sequence, the F0 chimeric mouse and a tissue-specific Cre tool mouse are hybridized to obtain an F1-generation knockout heterozygote, and finally, the F1-generation heterozygote is subjected to inbreeding to obtain an IGFBP7 gene knockout homozygote mouse. The invention provides a good basis for the research and experience verification of the IGFBP7 gene function, and has great significance for perfecting the muscle development regulation network.

Description

technical field [0001] The invention relates to the fields of genetics and biotechnology, and relates to an IGFBP7 muscle tissue-specific knockout mouse animal model and a method for constructing the gene knockout mouse model. Background technique [0002] The growth and development of muscle directly affects the life activities of the body, and in addition, it is directly related to the meat production traits in livestock and poultry production. Therefore, elucidating the molecular mechanism of key candidate genes of muscle growth and development and enriching the molecular regulatory network of muscle growth and development can provide important therapeutic targets for human muscle growth and development diseases on the one hand, and accelerate the progress of molecular breeding of livestock and poultry on the other hand. [0003] Insulin-like growth factor binding protein-7 (IGFBP7), also known as mac25 and IGFPB-rP1, is a secreted protein of the IGFBP family. Since the ...

Claims

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Application Information

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IPC IPC(8): C12N15/90C12N15/85C12N15/12A01K67/027A61K49/00
CPCC07K14/4743C12N15/907C12N15/8509A01K67/0276A61K49/0008C12N2800/107C12N2800/30A01K2217/075A01K2227/105A01K2267/03A01K2267/0393Y02A40/70
Inventor 方美英雒亚彪产舒恒薛明明肖莲梅白莹许桥汤启国
Owner CHINA AGRI UNIV
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