Coal rock liquid phase flow biological nucleic acid probe tracing method

Abstract

The invention discloses a coal rock liquid phase flow biological nucleic acid probe tracing method, and belongs to a coal rock stratum hydraulic tracing test evaluation method. An artificially edited fluorescent protein expression plasmid is converted into tracer bacteria to serve as a tracer medium, distribution of strains in mesopores is completed by means of free diffusion of the tracer bacteria along with liquid and single-layer adsorption characteristics of the tracer bacteria on the surface of a coal body, and the tracer bacteria carry specific green fluorescent protein nucleic acid probes. The nucleic acid probe is recombined and fused with PET22b expression plasmids and can be autonomously replicated and expressed along with the growth of tracer bacteria, qualitative and quantitative analysis of the distribution of the tracer bacteria is realized by a biological nucleic acid probe amplification method, and the trend of a coal seam fracture system is accurately judged. The method has the advantages that escherichia coli is used as a tracing medium; 5 [mu] m), which cannot be continuously segmented, carries a special easily-identified DNA fragment, has a fluorescence characteristic, can realize concentration of thalli through centrifugation, and has a sensitive factor autonomous avoidance characteristic.

Description

Technical field [0001] The present invention relates to a hydraulic tracer test evaluation method for coal rock formations, in particular a coal-rock liquid flow bionucleic acid probe tracing method. Background [0002] Hydraulic measures (hydraulic fracturing, coal seam water injection, etc.) are effective technical ways to increase the permeability of coal rock mass, promote gas desorption, and improve the extraction effect of coal seam methane, which has the advantages of large impact and significant transparency enhancement effect, and is widely used in the permeability of low permeability coal seam. However, due to the colorless and odorless characteristics of water and the opaque coal rock mass, it is impossible to clearly determine the flow direction, scope of influence, and its constraints of injected water during the implementation of hydraulic measures technology, resulting in great uncertainty and uncontrollability in the implementation of hydraulic measures. [0003] ...

AI Technical Summary

Problems solved by technology

Gas tracer solves the problem of interference between coalbed water and coalbed components, but there are still two technical problems in this method: ① Coalbed methane has a dilution and interference effect on the tracer gas. When the tracer gas is excessively diluted by coalbed methane, The test and analysis of tracer gas becomes more difficult, thus reducing the sensitivity of tracer technology; (2) Affected by the water sealing effect in coal mesopore fracture space, it is difficult for tracer gas to effectively distribute in mesopore fracture space, so the gas tracer It can only be used for the analysis of the penetration of large fissures, not for the evaluation of hydraulic measures

[0007] Based on the above test technologies, the existing hydraulic test and evaluation technologies generally have technical problems of low reliability, low recognition, complicated operation, and high cost

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